University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Resveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis.

Shakibaei, M, Csaki, C, Nebrich, S and Mobasheri, A (2008) Resveratrol suppresses interleukin-1beta-induced inflammatory signaling and apoptosis in human articular chondrocytes: potential for use as a novel nutraceutical for the treatment of osteoarthritis. Biochem Pharmacol, 76 (11). pp. 1426-1439.

Full text not available from this repository.

Abstract

Osteoarthritis is an inflammatory disease of load-bearing synovial joints that is currently treated with drugs that exhibit numerous side effects and are only temporarily effective on pain, the main symptom of the disease. Consequently, there is an acute need for novel, safe and more effective chemotherapeutic agents for the treatment of osteoarthritis and related arthritic diseases. Resveratrol is a phytoalexin stilbene produced naturally by plants including red grapes, peanuts and various berries. Recent research in various cell models has demonstrated that resveratrol is safe and has potent anti-inflammatory properties. However, its potential for treating arthritic conditions has not been explored. In this study we provide experimental evidence that resveratrol inhibits the expression of VEGF, MMP-3, MMP-9 and COX-2 in human articular chondrocytes stimulated with the pro-inflammatory cytokine IL-1beta. Since these gene products are regulated by the transcription factor NF-kappaB, we investigated the effects of resveratrol on IL-1beta-induced NF-kappaB signaling pathway. Resveratrol, like N-Ac-Leu-Leu-norleucinal (ALLN) suppressed IL-1beta-induced proteasome function and the degradation of IkappaBalpha (an inhibitor of NF-kappaB) without affecting IkappaBalpha kinase activation, IkappaBalpha-phosphorylation or IkappaBalpha-ubiquitination which suppressed nuclear translocation of the p65 subunit of NF-kappaB and its phosphorylation. Furthermore, we observed that resveratrol as well as ALLN inhibited IL-1beta-induced apoptosis, caspase-3 activation and PARP cleavage in human articular chondrocytes. In summary, our results suggest that resveratrol suppresses apoptosis and inflammatory signaling through its actions on the NF-kappaB pathway in human chondrocytes. We propose that resveratrol should be explored further for the prophylactic treatment of osteoarthritis in humans and companion animals.

Item Type: Article
Authors :
NameEmailORCID
Shakibaei, MUNSPECIFIEDUNSPECIFIED
Csaki, CUNSPECIFIEDUNSPECIFIED
Nebrich, SUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Date : 1 December 2008
Identification Number : 10.1016/j.bcp.2008.05.029
Uncontrolled Keywords : Apoptosis, Blotting, Western, Cartilage, Articular, Cell Nucleus, Cells, Cultured, Chondrocytes, Dietary Supplements, Dose-Response Relationship, Drug, Extracellular Matrix, Humans, Inflammation Mediators, Interleukin-1beta, Microscopy, Electron, Transmission, NF-kappa B, Osteoarthritis, Protein Transport, Signal Transduction, Stilbenes
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:12
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826805

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800