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Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection.

Graham, NS, Clutterbuck, AL, James, N, Lea, RG, Mobasheri, A, Broadley, MR and May, ST (2010) Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection. Vet J, 186 (3). pp. 323-327.

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Abstract

Affymetrix GeneChip arrays are a powerful tool for transcriptome profiling and have been applied to a wide range of species. A genomic DNA (gDNA)-based probe selection method has been developed which broadens the range of species to which GeneChips may be successfully applied. This study demonstrated that gDNA-based probe selection on the Affymetrix U133+2 GeneChip array can be used to study the equine transcriptome which, to date, has received only limited attention. More than 29,000 transcripts can be detected in equine brain and liver and in primary cultures of equine articular chondrocytes. Gene ontology analysis of differentially expressed genes revealed the presence of expected categories within each tissue. The level of gene expression could also be correlated with the phenotypes and specialised functions of each tissue. The results demonstrated that probe selection on a human chip can be successfully used to study the equine transcriptome.

Item Type: Article
Authors :
NameEmailORCID
Graham, NSUNSPECIFIEDUNSPECIFIED
Clutterbuck, ALUNSPECIFIEDUNSPECIFIED
James, NUNSPECIFIEDUNSPECIFIED
Lea, RGUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Broadley, MRUNSPECIFIEDUNSPECIFIED
May, STUNSPECIFIEDUNSPECIFIED
Date : December 2010
Identification Number : 10.1016/j.tvjl.2009.08.030
Uncontrolled Keywords : Animals, DNA, DNA Probes, Gene Expression, Genome, Horses, Humans, Oligonucleotide Array Sequence Analysis, Selection, Genetic, Species Specificity, Transcription, Genetic
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826794

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