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Glucose transporter Glut-1 is detectable in peri-necrotic regions in many human tumor types but not normal tissues: Study using tissue microarrays.

Airley, R, Evans, A, Mobasheri, A and Hewitt, SM (2010) Glucose transporter Glut-1 is detectable in peri-necrotic regions in many human tumor types but not normal tissues: Study using tissue microarrays. Ann Anat, 192 (3). pp. 133-138.

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Abstract

The hypoxic tumor microenvironment is associated with malignant progression and poor treatment response. The glucose transporter Glut-1 is a prognostic factor and putative hypoxia marker. So far, studies of Glut-1 in cancer have utilized conventional immunohistochemical analysis in a series of individual biopsy or surgical specimens. Tissue microarrays, however, provide a rapid, inexpensive means of profiling biomarker expression. To evaluate hypoxia markers, tissue cores must show the architectural features of hypoxia; i.e. viable tissue surrounding necrotic regions. Glut-1 may be a useful biomarker to validate tissue microarrays for use in studies of hypoxia-regulated genes in cancer. In this study, we carried out immunohistochemical detection of Glut-1 protein in many tumor and normal tissue types in a range of tissue microarrays. Glut-1 was frequently found in peri-necrotic regions, occurring in 9/34 lymphomas, 6/12 melanomas, and 5/16 glioblastomas; and in 43/54 lung, 22/84 colon, and 23/60 ovarian tumors. Expression was rare in breast (6/40) and prostate (1/57) tumors, and in normal tissue, was restricted to spleen, tongue, and CNS endothelium. In conclusion, tissue microarrays enable the observation of Glut-1 expression in peri-necrotic regions, which may be linked to hypoxia, and reflect previous studies showing differential Glut-1 expression across tumor types and non-malignant tissue.

Item Type: Article
Authors :
NameEmailORCID
Airley, RUNSPECIFIEDUNSPECIFIED
Evans, AUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Hewitt, SMUNSPECIFIEDUNSPECIFIED
Date : 20 May 2010
Identification Number : https://doi.org/10.1016/j.aanat.2010.03.001
Uncontrolled Keywords : Anoxia, Biomarkers, Breast Neoplasms, Colonic Neoplasms, Disease Progression, Female, Gene Expression Regulation, Neoplastic, Glucose Transporter Type 1, Humans, Immunohistochemistry, Lung Neoplasms, Necrosis, Neoplasms, Organ Specificity, Ovarian Neoplasms, Prognosis, Protein Array Analysis, Reference Values
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826782

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