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Neuronal responses in the brainstem and hypothalamic nuclei following insulin treatment during the late follicular phase in the ewe.

Ghuman, SP, Morris, R, Scherzer, J, Routly, JE, Mobasheri, A, Smith, RF and Dobson, H (2011) Neuronal responses in the brainstem and hypothalamic nuclei following insulin treatment during the late follicular phase in the ewe. Reprod Domest Anim, 46 (1). pp. 121-129.

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Abstract

The aim of this study was to determine the neuronal responses following insulin administration during the late follicular phase. Intact ewes were given either saline or insulin (5 IU/kg, i.v.) at 35 h after progesterone withdrawal and killed 3 h later. There was a marked increase in the number of Fos-positive noradrenergic neurones in the caudal brainstem of insulin-treated ewes. In the hypothalamic paraventricular nucleus, insulin treatment increased the presence of Fos-positive corticotrophin-releasing hormone neurones (from 2% to 98%) and Fos-positive arginine vasopressin parvocellular neurones (from 2% to 46%). Interestingly, after insulin treatment, despite a general increase in Fos-positive neurones in the arcuate nucleus (ARC), there was a marked reduction (from 47% to 1%) in Fos-positive β-endorphin neurones. Similarly, colocalized Fos and oestradiol receptor (ER) α-positive neurones decreased in the ARC after insulin (from 7% to 3%). Conversely, in the ventromedial nucleus, ERα-positive neurones with Fos increased (from 7% to 22%) alongside a general increase in Fos-positive neurones. Overall, a complex system of neurones in brainstem and hypothalamus is activated following insulin administration during the late follicular phase.

Item Type: Article
Authors :
NameEmailORCID
Ghuman, SPUNSPECIFIEDUNSPECIFIED
Morris, RUNSPECIFIEDUNSPECIFIED
Scherzer, JUNSPECIFIEDUNSPECIFIED
Routly, JEUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Smith, RFUNSPECIFIEDUNSPECIFIED
Dobson, HUNSPECIFIEDUNSPECIFIED
Date : February 2011
Identification Number : https://doi.org/10.1111/j.1439-0531.2010.01605.x
Uncontrolled Keywords : Animals, Arcuate Nucleus of Hypothalamus, Arginine Vasopressin, Brain Stem, Cell Count, Corticotropin-Releasing Hormone, Estrogen Receptor alpha, Female, Follicular Phase, Hypothalamus, Insulin, Neurons, Paraventricular Hypothalamic Nucleus, Proto-Oncogene Proteins c-fos, Sheep, beta-Endorphin
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826781

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