Hypoxia and a hypoxia mimetic up-regulate matrix metalloproteinase 2 and 9 in equine laminar keratinocytes.

Medina-Torres, CE, Mason, SL, Floyd, RV, Harris, PA and Mobasheri, A (2011) Hypoxia and a hypoxia mimetic up-regulate matrix metalloproteinase 2 and 9 in equine laminar keratinocytes. Vet J, 190 (2). e54-e59.

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Abstract

The aim of this study was to determine if hypoxia and the hypoxia mimetic cobalt chloride regulate the activity of matrix metalloproteinase (MMP)-2 and -9 in cultures of equine hoof keratinocytes. These effects were assessed in primary cultures of laminar keratinocytes using gelatin zymography. Incubation of keratinocytes with cobalt chloride significantly increased the levels of active MMP-2 compared to untreated controls. Hypoxia significantly increased the expression of active MMP-2 and -9 in keratinocyte cultures. This up-regulation was observed after 6h and peaked at 24h. The study findings provide novel evidence of a potential link between hypoxia within the hoof and up-regulation of MMPs which may in turn result in damage to the lamellar basement membrane.

Item Type:	Article
Authors :	Name Email ORCID Medina-Torres, CE Mason, SL Floyd, RV Harris, PA Mobasheri, A a.mobasheri@surrey.ac.uk
Date :	November 2011
DOI :	10.1016/j.tvjl.2011.02.026
Uncontrolled Keywords :	Animals, Anoxia, Biomimetics, Cell Hypoxia, Cells, Cultured, Cobalt, Foot Diseases, Hoof and Claw, Horse Diseases, Horses, Keratinocytes, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Up-Regulation
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:11
Last Modified :	16 Jan 2019 17:55
URI:	http://epubs.surrey.ac.uk/id/eprint/826774

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