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Anti-inflammatory and anti-catabolic effects of TENDOACTIVE® on human tenocytes in vitro.

Shakibaei, M, Buhrmann, C and Mobasheri, A (2011) Anti-inflammatory and anti-catabolic effects of TENDOACTIVE® on human tenocytes in vitro. Histol Histopathol, 26 (9). pp. 1173-1185.

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Tendons have a limited capacity for self-repair due to the low density and mitotic activity of tenocytes. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) have been identified as the main initiators of tendinopathies, stimulating inflammation, apoptosis and extracellular matrix (ECM) degradation. The aim of this study was to evaluate the potential of Tendoactive®, a newly developed proprietary nutraceutical formulation that includes mucopolysaccharides, collagen and vitamin C, in an in vitro model of tendon inflammation. The effects of Tendoactive® were studied in primary cultures of human tenocytes treated with IL-1β for up to 72 h. Expression of collagen type I, integrin β1, cyclo-oxygenase-2 (COX-2), caspase-3 and matrix metalloproteinase-1 (MMP-1) was monitored by western blotting. The effects of Tendoactive® on the expression, phosphorylation and nuclear translocation of protein components of the NF-κB system were studied by western blotting and immunofluorescence respectively. Treatment of tenocytes with Tendoactive® suppressed IL-1β-induced NF-κB activation and p65 nuclear translocation. These events correlated with down-regulation of NF-κB targets including COX-2, MMP-1 and activated caspase-3. Tendoactive® also reversed the IL-1β-induced down-regulation of collagen type I and β1-integrin receptor expression. These results indicate that Tendoactive® has nutraceutical potential as an anti-inflammatory agent for treating tendinopathy through suppression of NF-κB mediated IL-1β catabolic signalling pathways in tenocytes.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Shakibaei, M
Buhrmann, C
Date : September 2011
Uncontrolled Keywords : Adult, Anti-Inflammatory Agents, Non-Steroidal, Apoptosis, Blotting, Western, Cell Nucleus, Cell Proliferation, Cell Separation, Cell Survival, Cells, Cultured, Chondrocytes, Culture Media, Dietary Supplements, Fluorescent Antibody Technique, Humans, Interleukin-1beta, Male, Metabolism, Microscopy, Electron, Transmission, Middle Aged, Mitochondria, NF-kappa B, Signal Transduction, Tendons, Transcription Factor RelA
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 24 Jan 2020 18:40

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