University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Immunolocalization of aquaporin 5 during rat ovarian follicle development and expansion of the preovulatory cumulus oophorus.

Starowicz, A, Grzesiak, M, Mobasheri, A and Szoltys, M (2014) Immunolocalization of aquaporin 5 during rat ovarian follicle development and expansion of the preovulatory cumulus oophorus. Acta Histochem, 116 (3). pp. 457-465.

Full text not available from this repository.

Abstract

Immunofluorescent localization of aquaporin 5 (AQP5) was investigated in rat ovarian follicles during development and preovulatory cumulus oophorus expansion. Ampullary cumuli oophori complexes (COCs) were examined. Analysis revealed that AQP5 immunostaining appeared in preantral follicles and formed a characteristic ring encircling and touching the oolemma. The staining represented most likely AQP5 functioning at the ends of corona radiata cell projections, anchoring on the oocyte surface. However, several hours after the presumptive preovulatory LH surge, when the process of expansion of COCs started, the AQP5 staining appeared also on the cumulus granulosa cells and in the extracellular matrix. In the postovulatory ampullary COCs the fluorescent ring was not observed, which may be the result of the well-established preovulatory withdrawal of projections from the zona pellucida. At that time-point immunofluorescent staining of AQP5 appeared in most oocytes and was also present in the apical membrane of epithelial cells of the oviduct ampulla. The latter observation suggests that after ovulation AQP5 is involved in the transcellular movement of water in the oviduct ampulla and oocytes in rats.

Item Type: Article
Authors :
NameEmailORCID
Starowicz, AUNSPECIFIEDUNSPECIFIED
Grzesiak, MUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Szoltys, MUNSPECIFIEDUNSPECIFIED
Date : April 2014
Identification Number : https://doi.org/10.1016/j.acthis.2013.10.001
Uncontrolled Keywords : Aquaporin 5, Cumulus-oocyte complex, Follicle, Ovary, Rat, Animals, Aquaporin 5, Cumulus Cells, Female, Oocytes, Organ Specificity, Ovarian Follicle, Ovulation, Protein Transport, Rats, Wistar
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826756

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800