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Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model.

Lin, A, Jimenez, J, Derr, J, Vera, P, Manapat, ML, Esvelt, KM, Villanueva, L, Liu, DR and Chen, IA (2011) Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. PLoS One, 6 (5).

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Abstract

Conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. Strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. Filamentous bacteriophages were first observed to inhibit conjugation several decades ago. Here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugative pilus by phage particles. This interaction is mediated primarily by phage coat protein g3p, and exogenous addition of the soluble fragment of g3p inhibited conjugation at low nanomolar concentrations. Our data are quantitatively consistent with a simple model in which association between the pili and phage particles or g3p prevents transmission of an F plasmid encoding tetracycline resistance. We also observe a decrease in the donor ability of infected cells, which is quantitatively consistent with a reduction in pili elaboration. Since many antibiotic-resistance factors confer susceptibility to phage infection through expression of conjugative pili (the receptor for filamentous phage), these results suggest that phage may be a source of soluble proteins that slow the spread of antibiotic resistance genes.

Item Type: Article
Authors :
NameEmailORCID
Lin, AUNSPECIFIEDUNSPECIFIED
Jimenez, Jj.jimenez@surrey.ac.ukUNSPECIFIED
Derr, JUNSPECIFIEDUNSPECIFIED
Vera, PUNSPECIFIEDUNSPECIFIED
Manapat, MLUNSPECIFIEDUNSPECIFIED
Esvelt, KMUNSPECIFIEDUNSPECIFIED
Villanueva, LUNSPECIFIEDUNSPECIFIED
Liu, DRUNSPECIFIEDUNSPECIFIED
Chen, IAUNSPECIFIEDUNSPECIFIED
Date : 2011
Identification Number : 10.1371/journal.pone.0019991
Uncontrolled Keywords : Bacteriophage M13, Conjugation, Genetic, Escherichia coli, F Factor, Genes, Viral, Models, Biological, Pili, Sex, Time Factors, Viral Proteins, Virus Replication
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:11
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826732

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