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Purification and analyses of the specificity of two putative diagnostic antigens for larval cyathostomin infection in horses.

Dowdall, SM, Proudman, CJ, Love, S, Klei, TR and Matthews, JB (2003) Purification and analyses of the specificity of two putative diagnostic antigens for larval cyathostomin infection in horses. Res Vet Sci, 75 (3). pp. 223-229.

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Abstract

Cyathostomins are important equine gastrointestinal parasites. Mass emergence of mucosal stage larvae causes a potentially fatal colitis. Mucosal stages are undetectable non-invasively. An assay that would estimate mucosal larval stage infection would greatly assist in treatment, control and prognosis. Previously, we identified two putative diagnostic antigens (20 and 25 kDa) in somatic larval preparations. Here, we describe their purification and antigen-specific IgG(T) responses to them. Western blots confirmed the purity of the antigens and showed that epitopes in the 20 kDa complex were specific to larval cyathostomins. No cross-reactive antigens appeared to be present in Parascaris equorum or Strongyloides westeri species. Low levels of cross-reactivity were observed in Strongylus edentatus and Strongylus vulgaris species. Use of purified antigens greatly reduced background binding in equine sera. These results indicate that both antigen complexes may be of use in a diagnostic assay.

Item Type: Article
Authors :
NameEmailORCID
Dowdall, SMUNSPECIFIEDUNSPECIFIED
Proudman, CJc.proudman@surrey.ac.ukUNSPECIFIED
Love, SUNSPECIFIEDUNSPECIFIED
Klei, TRUNSPECIFIEDUNSPECIFIED
Matthews, JBUNSPECIFIEDUNSPECIFIED
Date : December 2003
Uncontrolled Keywords : Animals, Antibodies, Helminth, Antigens, Helminth, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Epitopes, Horses, Immunoglobulin G, Intestinal Diseases, Parasitic, Species Specificity, Strongyle Infections, Equine, Strongylida Infections, Strongylus
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:10
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826687

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