Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever.

Sánchez-Cordón, PJ, Romanini, S, Salguero, FJ, Núñez, A, Bautista, MJ, Jover, A and Gómez-Villamos, JC (2002) Apoptosis of thymocytes related to cytokine expression in experimental classical swine fever. J Comp Pathol, 127 (4). pp. 239-248.

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Abstract

Atrophy of the thymic cortex and loss of thymocytes were studied in 32 pigs inoculated with the virulent strain "Alfort" of classical swine fever (CSF) virus and killed at intervals from 2 to 15 days after infection. Immunohistochemical, ultrastructural, ELISA and TUNEL methods were used. The results suggested that direct action of CSF virus on thymocytes played no more than a minor role. The massive lymphoid depletion observed in the thymus, may, however, have been associated with the numerical increase in monocytes-macrophages in this organ, and their secretory activation, leading to synthesis and release of tumour necrosis factor (TNF)-alpha, interleukin (IL)-1alpha and C1q complement component as main chemical mediators, and IL-1beta and IL-6 as minor mediators. These cytokines (TNF-alpha and IL-1alpha) may have played a role in the apoptosis of thymocytes, demonstrated by TUNEL and ultrastructural methods. The pathogenetic mechanism outlined may contribute to the lymphoid depletion observed in others organs in CSF and may explain the lymphopenia characteristic of the disease.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Sánchez-Cordón, PJ, Romanini, S, Salguero, FJ, Núñez, A, Bautista, MJ, Jover, A and Gómez-Villamos, JC
Date :	November 2002
Uncontrolled Keywords :	Animals, Apoptosis, Cell Count, Classical Swine Fever, Classical swine fever virus, Cytokines, Enzyme-Linked Immunosorbent Assay, Female, Immunohistochemistry, In Situ Nick-End Labeling, Macrophages, Male, Swine, Thymus Gland, Time Factors
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:08
Last Modified :	24 Jan 2020 18:33
URI:	http://epubs.surrey.ac.uk/id/eprint/826573

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