University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Classical Swine Fever: pathology of bone marrow.

Gomez-Villamandos, JC, Salguero, FJ, Ruiz-Villamor, E, Sánchez-Cordón, PJ, Bautista, MJ and Sierra, MA (2003) Classical Swine Fever: pathology of bone marrow. Vet Pathol, 40 (2). pp. 157-163.

Full text not available from this repository.

Abstract

Twenty pigs were inoculated with a virulent isolate (Quillota strain) of classical swine fever (CSF) virus to determine the chronological development of lesions in bone marrow. Histopathologic, ultrastructural and immunohistochemical (detection of viral antigen gp55, myeloid-histiocyte antigen, CD3 antigen, and FVIII-rag), and morphometric techniques were employed. Viral antigen was detected from 2 days postinfection (dpi) in stromal and haematopoitic cells, and severe atrophy related to apoptosis of haematopoitic cells was observed. Megakaryocytes (MKs) did not show significant changes in number, but there were important qualitative changes including 1) increased numbers of cloud-nuclei MKs, microMKs, apoptotic MKs, and atypical nucleated MKs and 2) decreased number of typical nucleated MKs. Morphometric study of these cells showed a decrease in cytoplasmic area. MK infection was detected from 2 dpi, but in a small percentage of cells. Myeloid cells showed quantitative changes, with an increase in granulocyte numbers. Apoptosis of lymphocytes and viral infection of erythroblasts were also observed. The main changes in stroma were depletion of T lymphocytes in the middle phase of the experiment and macrophages. Viral infection was also observed in these cells. MK lesions suggest dysmegakaryocytopoiesis, which would aggravate the thrombocytopenia already present and could be responsible for it. Granulocyte changes would lead to the appearance of circulating immature forms, whereas lymphocyte apoptosis in bone marrow would contribute to lymphopenia.

Item Type: Article
Authors :
NameEmailORCID
Gomez-Villamandos, JCUNSPECIFIEDUNSPECIFIED
Salguero, FJUNSPECIFIEDUNSPECIFIED
Ruiz-Villamor, EUNSPECIFIEDUNSPECIFIED
Sánchez-Cordón, PJUNSPECIFIEDUNSPECIFIED
Bautista, MJUNSPECIFIEDUNSPECIFIED
Sierra, MAUNSPECIFIEDUNSPECIFIED
Date : March 2003
Uncontrolled Keywords : Animals, Antigens, CD3, Antigens, Viral, Apoptosis, Bone Marrow, Classical Swine Fever, Classical swine fever virus, Female, Granulocytes, Immunohistochemistry, Leukocyte Count, Male, Megakaryocytes, Microscopy, Electron, Platelet Count, Swine, von Willebrand Factor
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:08
Last Modified : 17 May 2017 10:08
URI: http://epubs.surrey.ac.uk/id/eprint/826571

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800