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Immunohistochemical detection of the expression of pro-inflammatory cytokines by ovine pulmonary macrophages.

Carrasco, L, Núñez, A, Sánchez-Cordón, PJ, Pedrera, M, Fernández de Marco, M, Salguero, FJ and Gómez-Villamandos, JC (2004) Immunohistochemical detection of the expression of pro-inflammatory cytokines by ovine pulmonary macrophages. J Comp Pathol, 131 (4). pp. 285-293.

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The aim of this study was to determine the expression of three proinflammatory cytokines by pulmonary macrophages of sheep in paraffin wax-embedded tissue. Samples of lung from seven healthy sheep were fixed by immersion in either 10% neutral buffered formalin, acetic formalin, paraformaldehyde-lysine-periodate or Bouin's solution and processed for structural and immunohistochemical studies. The expression of interleukin (IL)-1alpha, IL-6 and tumour necrosis factor (TNF)-alpha by pulmonary intravascular macrophages (PIMs) and alveolar macrophages (AMs) was detected by the avidin-biotin-peroxidase (ABC) technique. Bouin's solution proved to be the most suitable fixative and Tween 20 the most effective pretreatment for increasing permeability. Constitutive expression of IL-1alpha, IL-6 and TNF-alpha by both macrophage populations was detected. The number of PIMs expressing IL-1alpha (the predominant cytokine in ovine lung) was higher than that of AMs, while the expression of IL-6 was greater in AMs. No differences between PIMS and AMs were found in respect of TNF-alpha expression. The evaluation of cytokine expression represents a valuable tool for studying the pathogenesis of disease in the ovine lung.

Item Type: Article
Authors :
Carrasco, L
Núñez, A
Sánchez-Cordón, PJ
Pedrera, M
Fernández de Marco, M
Salguero, FJ
Gómez-Villamandos, JC
Date : November 2004
DOI : 10.1016/j.jcpa.2004.05.004
Uncontrolled Keywords : Animals, Cell Membrane Permeability, Cytokines, Fixatives, Immunoenzyme Techniques, Interleukin-1, Interleukin-6, Macrophages, Alveolar, Polysorbates, Sheep, Tissue Fixation, Tumor Necrosis Factor-alpha
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:08
Last Modified : 17 May 2017 10:08

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