Expression of proinflammatory cytokines by hepatic macrophages in acute classical swine fever.

Núñez, A, Gómez-Villamandos, JC, Sánchez-Cordón, PJ, Fernández de Marco, M, Pedrera, M, Salguero, FJ and Carrasco, L (2005) Expression of proinflammatory cytokines by hepatic macrophages in acute classical swine fever. J Comp Pathol, 133 (1). pp. 23-32.

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Abstract

Fourteen pigs were inoculated with the 'Alfort 187' strain of classical swine fever (CSF) virus and killed in pairs at 2, 4, 7, 9, 11, 14 or 17 days post-inoculation for histopathological, ultrastructural and immunohistochemical examination. For the latter method, the antibodies used were those against viral antigen Gp55, porcine myeloid marker SWC3, IL-1alpha, IL-6, TNF-alpha and Factor VIII-related antigen. Activation and increase in the number of hepatic macrophages was observed following viral detection in liver, as well as an increase in IL-1alpha and IL-6 production, mainly by Kupffer cells. Maximum detection of viral antigen was observed in the middle stage of the experiment coinciding with overexpression of the three cytokines studied, with IL-6 production by interstitial macrophages prominent at the end. Additionally, the labelling of platelets for Factor VIII-related antigen and the ultrastructural study of the sinusoids revealed activation and aggregation of thrombocytes close to Kupffer cells at the beginning of the infection. The liver seems to play a prominent role in the origin of the thrombocytopenia that occurs in CSF and contributes to the overexpression of proinflammatory cytokines considered responsible for the disorders observed during the course of the disease.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Núñez, A, Gómez-Villamandos, JC, Sánchez-Cordón, PJ, Fernández de Marco, M, Pedrera, M, Salguero, FJ and Carrasco, L
Date :	July 2005
DOI :	10.1016/j.jcpa.2005.01.003
Uncontrolled Keywords :	Animals, Classical Swine Fever, Classical swine fever virus, Cytokines, Female, Immunohistochemistry, Kupffer Cells, Liver, Macrophage Activation, Male, Microscopy, Electron, Transmission, Swine
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:08
Last Modified :	24 Jan 2020 18:33
URI:	http://epubs.surrey.ac.uk/id/eprint/826559

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