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Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine.

Díaz-San Segundo, F, Salguero, FJ, de Avila, A, de Marco, MM, Sánchez-Martín, MA and Sevilla, N (2006) Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine. J Virol, 80 (5). pp. 2369-2379.

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Abstract

Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious vesicular disease of cloven-hoofed animals. In the present study we use FMDV serotype C infection of swine to determine, by analytical techniques, the direct ex vivo visualization of virus-infected immune cells during the first 17 days of infection. We report, for the first time, that FMDV C-S8c1 can infect T and B cells at short periods of time postinoculation, corresponding with the peak of the viremia. There is a significant lymphopenia that involves CD3(+) CD4(-) CD8(+/-), CD3(+) CD4(-) CD8(+)Tc, and CD3(+) CD4(+) CD8(+) memory Th but not CD3(+) CD4(+) CD8(-) naïve Th lymphocytes. In addition, a profound depletion of the vast majority of peripheral T cells in lymph nodes and spleen is observed. This selective depletion of T cells is not due mainly to in situ death via apoptosis as visualized by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) technique. Thus, early infection of T cells by FMDV may be the main cause of the observed T-cell depletion. Importantly, this lack of T cells is reflected in a reduced response to mitogen activation, which in many cases is totally eliminated. These data suggest a mechanism by which the virus causes a transient immunosuppression, subvert the immune systems, and spreads. These results have important implications for our understanding of early events in the development of a robust immune response against FMDV.

Item Type: Article
Authors :
NameEmailORCID
Díaz-San Segundo, FUNSPECIFIEDUNSPECIFIED
Salguero, FJUNSPECIFIEDUNSPECIFIED
de Avila, AUNSPECIFIEDUNSPECIFIED
de Marco, MMUNSPECIFIEDUNSPECIFIED
Sánchez-Martín, MAUNSPECIFIEDUNSPECIFIED
Sevilla, NUNSPECIFIEDUNSPECIFIED
Date : March 2006
Identification Number : https://doi.org/10.1128/JVI.80.5.2369-2379.2006
Uncontrolled Keywords : Animals, Apoptosis, B-Lymphocytes, Female, Foot-and-Mouth Disease, Foot-and-Mouth Disease Virus, Immune Tolerance, In Situ Nick-End Labeling, Lymph Nodes, Lymphocyte Activation, Lymphocyte Count, Lymphopenia, Spleen, Swine, Swine Diseases, T-Lymphocyte Subsets, T-Lymphocytes
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:08
Last Modified : 17 May 2017 10:08
URI: http://epubs.surrey.ac.uk/id/eprint/826555

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