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Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4.

Munera, D, Ritchie, JM, Hatzios, SK, Bronson, R, Fang, G, Schadt, EE, Davis, BM and Waldor, MK (2014) Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun, 5.

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Abstract

The outbreak of diarrhoea and haemolytic uraemic syndrome that occurred in Germany in 2011 was caused by a Shiga toxin-producing enteroaggregative Escherichia coli (EAEC) strain. The strain was classified as EAEC owing to the presence of a plasmid (pAA) that mediates a characteristic pattern of aggregative adherence on cultured cells, the defining feature of EAEC that has classically been associated with virulence. Here we describe an infant rabbit-based model of intestinal colonization and diarrhoea caused by the outbreak strain, which we use to decipher the factors that mediate the pathogen's virulence. Shiga toxin is the key factor required for diarrhoea. Unexpectedly, we observe that pAA is dispensable for intestinal colonization and development of intestinal pathology. Instead, chromosome-encoded autotransporters are critical for robust colonization and diarrhoeal disease in this model. Our findings suggest that conventional wisdom linking aggregative adherence to EAEC intestinal colonization is false for at least a subset of strains.

Item Type: Article
Authors :
NameEmailORCID
Munera, DUNSPECIFIEDUNSPECIFIED
Ritchie, JMj.ritchie@surrey.ac.ukUNSPECIFIED
Hatzios, SKUNSPECIFIEDUNSPECIFIED
Bronson, RUNSPECIFIEDUNSPECIFIED
Fang, GUNSPECIFIEDUNSPECIFIED
Schadt, EEUNSPECIFIEDUNSPECIFIED
Davis, BMUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Date : 20 January 2014
Identification Number : 10.1038/ncomms4080
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:06
Last Modified : 17 May 2017 14:47
URI: http://epubs.surrey.ac.uk/id/eprint/826362

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