Proteomic evaluation of cancer cells: Identification of cell surface proteins

Larkin, S and Aukim-Hastie, C (2011) Proteomic evaluation of cancer cells: Identification of cell surface proteins Methods in Molecular Biology, 731. pp. 395-405.

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Abstract

The plasma membrane proteome can be defined as the entire complement of proteins present in the plasma membrane at a specific time. The process of carcinogenesis leads to changes in the array of proteins present in the plasma membrane proteome. Analysis of differential expression of such proteins in cancer is extremely important; due to their position on the cell surface they have a potential for use as diagnostic and/or prognostic markers and therapeutic targets. Biotin labelling followed by avidin chromatography can be used to obtain membrane protein enriched lysates from cell lines, which can then be resolved using SDS-PAGE, coomassie staining and mass spectrometry. © 2011 Springer Science+Business Media, LLC.

Item Type:	Article
Authors :	Name Email ORCID Larkin, S UNSPECIFIED UNSPECIFIED Aukim-Hastie, C c.aukim-hastie@surrey.ac.uk UNSPECIFIED
Date :	1 January 2011
Identification Number :	10.1007/978-1-61779-080-5-32
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:04
Last Modified :	17 May 2017 10:04
URI:	http://epubs.surrey.ac.uk/id/eprint/826249

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