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Complement activation and liver impairment in trichloroethylene-sensitized BALB/c mice.

Zhang, J, Zha, W, Wang, F, Jiang, T, Xu, S, Yu, J, Zhou, C, Shen, T, Wu, C and Zhu, Q (2013) Complement activation and liver impairment in trichloroethylene-sensitized BALB/c mice. Int J Toxicol, 32 (6). pp. 431-441.

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Abstract

Our recent studies have shown that trichloroethylene (TCE) was able to induce multisystem injuries in the form of occupational medicamentosa-like dermatitis, including skin, kidney, and liver damages. However, the role of complement activation in the immune-mediated liver injury is not known. This study examined the role of complement activation in the liver injury in a mouse model of TCE-induced sensitization. Treatment of female BALB/c mice with TCE under specific dosing protocols resulted in skin inflammation and sensitization. Skin edema and erythema occurred in TCE-sensitized groups. Trichloroethylene sensitization produced liver histopathological lesions, increased serum alanine aminotransferase, aspartate transaminase activities, and the relative liver weight. The concentrations of serum complement components C3a-desArg, C5a-desArg, and C5b-9 were significantly increased in 24-hour, 48-hour, and 72-hour sensitization-positive groups treated with TCE and peaked in the 72-hour sensitization-positive group. Depositions of C3a, C5a, and C5b-9 into the liver tissue were also revealed by immunohistochemistry. Immunofluorescence further verified high C5b-9 expression in 24-hour, 48-hour, and 72-hour sensitization-positive groups in response to TCE treatment. Reverse transcription-polymerase chain reaction detected C3 messenger RNA expression in the liver, and this was significantly increased in 24-hour and 48-hour sensitization-positive groups with a transient reduction at 72 hours. These results provide the first experimental evidence that complement activation may play a key role in the generation and progression of immune-mediated hepatic injury by exposure to TCE.

Item Type: Article
Authors :
NameEmailORCID
Zhang, JUNSPECIFIEDUNSPECIFIED
Zha, WUNSPECIFIEDUNSPECIFIED
Wang, FUNSPECIFIEDUNSPECIFIED
Jiang, TUNSPECIFIEDUNSPECIFIED
Xu, SUNSPECIFIEDUNSPECIFIED
Yu, JUNSPECIFIEDUNSPECIFIED
Zhou, CUNSPECIFIEDUNSPECIFIED
Shen, TUNSPECIFIEDUNSPECIFIED
Wu, Cc.wu@surrey.ac.ukUNSPECIFIED
Zhu, QUNSPECIFIEDUNSPECIFIED
Date : November 2013
Identification Number : https://doi.org/10.1177/1091581813511337
Uncontrolled Keywords : C3a, C5a, C5b-9, complement activation, liver injury, trichloroethylene
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:03
Last Modified : 17 May 2017 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/826167

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