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Excretion of BSE and scrapie prions in stools from murine models

Maluquer de Motes, C, Grassi, J, Simon, S, Herva, ME, Torres, JM, Pumarola, M and Girones, R (2008) Excretion of BSE and scrapie prions in stools from murine models Vet Microbiol, 131 (1-2). pp. 205-211.

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Faeces from infected animals have been suggested as a potential source of contamination and transmission of prion diseases in the environment. This work describes the development of a procedure for the detection of PrP(res) in stools which is based on a detergent-based extraction and immunoprecipitation (IP). The procedure was evaluated by analyzing TSE-spiked sheep and mice faeces, and proved to be specific for PrP(res) with sensitivities of 5-10 microg of infected brain tissue. In order to analyze the shedding of prions, we studied stools from orally inoculated mice over 4-days post-inoculation and also stools from terminally sick scrapie-infected mice. PrP(res) was only detected in stools shortly after the oral ingestion of TSE agents. The procedure described could be a useful tool for studying the excretion of prions and for evaluating potential environmental contamination by prions.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Maluquer de Motes,
Grassi, J
Simon, S
Herva, ME
Torres, JM
Pumarola, M
Girones, R
Date : 18 September 2008
DOI : 10.1016/j.vetmic.2008.02.014
Uncontrolled Keywords : Animals, Blotting, Western, Cattle, Disease Models, Animal, Encephalopathy, Bovine Spongiform, Feces, Mice, Mice, Transgenic, PrPSc Proteins, Prions, Scrapie, Species Specificity, Time Factors
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:01
Last Modified : 24 Jan 2020 18:18

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