Isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian adenoviruses
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Maluquer de Motes, C, Hundesa, A, Almeida, FC, Bofill-Mas, S and Girones, R (2011) Isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian adenoviruses Virol J, 8.
Full text not available from this repository.Abstract
Adenoviruses of primates include human (HAdV) and simian (SAdV) isolates classified into 8 species (Human Adenovirus A to G, and Simian Adenovirus A). In this study, a novel adenovirus was isolated from a colony of cynomolgus macaques (Macaca fascicularis) and subcultured in VERO cells. Its complete genome was purified and a region encompassing the hexon gene, the protease gene, the DNA binding protein (DBP) and the 100 kDa protein was amplified by PCR and sequenced by primer walking. Sequence analysis of these four genes showed that the new isolate had 80% identity to other primate adenoviruses and lacked recombination events. The study of the evolutionary relationships of this new monkey AdV based on the combined sequences of the four genes supported a close relationship to SAdV-3 and SAdV-6, lineages isolated from Rhesus monkeys. The clade formed by these three types is separated from the remaining clades and establishes a novel branch that is related to species HAdV-A, F and G. However, the genetic distance corresponding to the newly isolated monkey AdV considerably differs from these as to belong to a new, not yet established species. Results presented here widen our knowledge on SAdV and represents an important contribution to the understanding of the evolutionary history of primate adenoviruses.
| Item Type: | Article | ||||||||||||||||||
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| Divisions : | Surrey research (other units) | ||||||||||||||||||
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| Date : | 2011 | ||||||||||||||||||
| DOI : | 10.1186/1743-422X-8-125 | ||||||||||||||||||
| Uncontrolled Keywords : | Adenoviruses, Simian, Animals, Cercopithecus aethiops, Evolution, Molecular, Humans, Macaca fascicularis, Molecular Sequence Data, Phylogeny, Vero Cells | ||||||||||||||||||
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| Depositing User : | Symplectic Elements | ||||||||||||||||||
| Date Deposited : | 17 May 2017 10:01 | ||||||||||||||||||
| Last Modified : | 24 Jan 2020 18:18 | ||||||||||||||||||
| URI: | http://epubs.surrey.ac.uk/id/eprint/826044 |
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