Heterologous extracellular production of enterocin P from Enterococcus faecium P13 in the methylotrophic bacterium Methylobacterium extorquens.

Gutiérrez, J, Bourque, D, Criado, R, Choi, YJ, Cintas, LM, Hernández, PE and Míguez, CB (2005) Heterologous extracellular production of enterocin P from Enterococcus faecium P13 in the methylotrophic bacterium Methylobacterium extorquens. FEMS Microbiol Lett, 248 (1). pp. 125-131.

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Abstract

Enterocin P (EntP), a strong antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, was produced by Methylobacterium extorquens. For heterologous expression of EntP in the methylotrophic bacterium M. extorquens, a recombinant plasmid was constructed. The gene encoding the EntP structural gene (entP) was cloned into the plasmid vector pCM80, under control of the methanol dehydrogenase promoter (P(mxaF)), to generate plasmid pS25. When M. extorquens ATCC 55366 was transformed with pS25, EntP was detected and quantified in supernatants of the recombinant M. extorquens S25 strain by using specific anti-EntP antibodies and a non-competitive indirect enzyme-linked immunosorbent assay (NCI-ELISA). Purification of EntP by hydrophobic adsorption and reverse-phase (RP-FPLC) chromatographies, permitted recovery of active EntP from the supernatants of M. extorquens S25 grown in a synthetic defined medium.

Item Type:	Article
Divisions :	Surrey research (other units)
Authors :	Gutiérrez, J, Bourque, D, Criado, R, Choi, YJ, Cintas, LM, Hernández, PE and Míguez, CB
Date :	1 July 2005
DOI :	10.1016/j.femsle.2005.05.029
Uncontrolled Keywords :	Bacteriocins, Enterococcus faecium, Methylobacterium extorquens, Plasmids, Recombinant Proteins
Related URLs :	Author
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 10:00
Last Modified :	24 Jan 2020 18:17
URI:	http://epubs.surrey.ac.uk/id/eprint/826018

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