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Identification of immunologically relevant proteins of Chlamydophila abortus using sera from experimentally infected pregnant ewes.

Marques, PX, Souda, P, O'Donovan, J, Gutierrez, J, Gutierrez, EJ, Worrall, S, McElroy, M, Proctor, A, Brady, C, Sammin, D, Basset, HF, Whitelegge, JP, Markey, BE and Nally, JE (2010) Identification of immunologically relevant proteins of Chlamydophila abortus using sera from experimentally infected pregnant ewes. Clin Vaccine Immunol, 17 (8). pp. 1274-1281.

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Abstract

Chlamydophila abortus is an intracellular pathogen and the etiological agent of enzootic abortion of ewes (EAE). C. abortus has a biphasic development cycle; extracellular infectious elementary bodies (EB) attach and penetrate host cells, where they give rise to intracellular, metabolically active reticulate bodies (RB). RB divide by binary fission and subsequently mature to EB, which, on rupture of infected cells, are released to infect new host cells. Pregnant ewes were challenged with 2 x 10(6) inclusion forming units (IFU) of C. abortus cultured in yolk sac (comprising both EB and RB). Serum samples were collected at 0, 7, 14, 21, 27, 30, 35, 40, and 43 days postinfection (dpi) and used to identify antigens of C. abortus expressed during disease. Additionally, sera from fetal lambs were collected at 30, 35, 40, and 43 dpi. All serum samples collected from experimentally infected pregnant ewes reacted specifically with several antigens of EB as determined by one-dimensional (1-D) and 2-D gel electrophoresis; reactive antigens identified by mass spectrometry included the major outer membrane protein (MOMP), polymorphic outer membrane protein (POMP), and macrophage infectivity potentiator (MIP) lipoprotein.

Item Type: Article
Authors :
NameEmailORCID
Marques, PXUNSPECIFIEDUNSPECIFIED
Souda, PUNSPECIFIEDUNSPECIFIED
O'Donovan, JUNSPECIFIEDUNSPECIFIED
Gutierrez, Jj.gutierrez@surrey.ac.ukUNSPECIFIED
Gutierrez, EJUNSPECIFIEDUNSPECIFIED
Worrall, SUNSPECIFIEDUNSPECIFIED
McElroy, MUNSPECIFIEDUNSPECIFIED
Proctor, AUNSPECIFIEDUNSPECIFIED
Brady, CUNSPECIFIEDUNSPECIFIED
Sammin, DUNSPECIFIEDUNSPECIFIED
Basset, HFUNSPECIFIEDUNSPECIFIED
Whitelegge, JPUNSPECIFIEDUNSPECIFIED
Markey, BEUNSPECIFIEDUNSPECIFIED
Nally, JEUNSPECIFIEDUNSPECIFIED
Date : August 2010
Identification Number : https://doi.org/10.1128/CVI.00163-10
Uncontrolled Keywords : Aborted Fetus, Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Blotting, Western, Chlamydophila, Chlamydophila Infections, Disease Models, Animal, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Female, Mass Spectrometry, Pregnancy, Pregnancy Complications, Infectious, Sheep, Sheep Diseases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:00
Last Modified : 17 May 2017 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/826003

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