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Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium.

Camelliti, P, Gallagher, JO, Kohl, P and McCulloch, AD (2006) Micropatterned cell cultures on elastic membranes as an in vitro model of myocardium. Nat Protoc, 1 (3). pp. 1379-1391.

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We describe here a new in vitro protocol for structuring cardiac cell cultures to mimic important aspects of the in vivo ventricular myocardial phenotype by controlling the location and mechanical environment of cultured cells. Microlithography is used to engineer microstructured silicon metal wafers. Those are used to fabricate either microgrooved silicone membranes or silicone molds for microfluidic application of extracellular matrix proteins onto elastic membranes (involving flow control at micrometer resolution). The physically or microfluidically structured membranes serve as a cell culture growth substrate that supports cell alignment and allows the application of stretch. The latter is achieved with a stretching device that can deliver isotropic or anisotropic stretch. Neonatal ventricular cardiomyocytes, grown on these micropatterned membranes, develop an in vivo-like morphology with regular sarcomeric patterns. The entire process from fabrication of the micropatterned silicon metal wafers to casting of silicone molds, microfluidic patterning and cell isolation and seeding takes approximately 7 days.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Gallagher, JO
Kohl, P
McCulloch, AD
Date : 2006
DOI : 10.1038/nprot.2006.203
Uncontrolled Keywords : Animals, Cell Culture Techniques, Elasticity, Extracellular Matrix Proteins, Heart Ventricles, Membranes, Artificial, Microfluidic Analytical Techniques, Myocardium, Rats, Silicon Compounds, Silicones
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 10:00
Last Modified : 24 Jan 2020 18:15

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