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Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation.

Mumford, AD, Chen, D, Dorling, A, Kemball-Cook, G and McVey, JH (2005) Generation of a polyclonal rabbit anti-mouse tissue factor antibody by nucleic acid immunisation. Thromb Haemost, 93 (1). pp. 160-164.

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Abstract

Tissue factor (TF) the cellular receptor and cofactor for factor VII, initiates coagulation and has also been implicated in several coagulation-independent functions, including inflammation, angiogenesis and tumour metastasis. Investigations of TF expression in mouse models of these processes has been limited by the availability of antibodies that specifically recognise mouse TF. We have generated a rabbit polyclonal antibody to mTF by DNA immunisation. This has yielded an antiserum that recognises native mTF in immunohistochemical and flow cytometric analyses. Furthermore, the antiserum is inhibitory in coagulation assays. This antiserum will be a valuable investigative tool in the analysis of mTF expression.

Item Type: Article
Authors :
NameEmailORCID
Mumford, ADUNSPECIFIEDUNSPECIFIED
Chen, DUNSPECIFIEDUNSPECIFIED
Dorling, AUNSPECIFIEDUNSPECIFIED
Kemball-Cook, GUNSPECIFIEDUNSPECIFIED
McVey, JHj.mcvey@surrey.ac.ukUNSPECIFIED
Date : January 2005
Identification Number : 10.1160/TH03-11-0703
Uncontrolled Keywords : Animals, Antibody Formation, Cloning, Molecular, DNA, Immune Sera, Immunization, Immunoassay, Mice, Monocytes, Rabbits, Thromboplastin
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:57
Last Modified : 17 May 2017 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/825822

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