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Adaptive response of equine intestinal Na+/glucose co-transporter (SGLT1) to an increase in dietary soluble carbohydrate.

Dyer, J, Al-Rammahi, M, Waterfall, L, Salmon, KS, Geor, RJ, Bouré, L, Edwards, GB, Proudman, CJ and Shirazi-Beechey, SP (2009) Adaptive response of equine intestinal Na+/glucose co-transporter (SGLT1) to an increase in dietary soluble carbohydrate. Pflugers Arch, 458 (2). pp. 419-430.

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Abstract

Experimental and epidemiological evidence suggests that consumption of hydrolyzable carbohydrate, hCHO (grain), by horses is an important risk factor for colic, a common cause of equine mortality. It is unknown whether the small intestinal capacity to digest hCHO and/or to absorb monosaccharides is limiting, or even if horses can adapt to increased carbohydrate load. We investigated changes in the brush-border membrane carbohydrate digestive enzymes and glucose absorptive capacity of horse small intestine in response to increased hCHO. Expression of the Na(+)/glucose co-transporter, SGLT1, was assessed by Western blotting, immunohistochemistry, Northern blotting, QPCR, and Na(+)-dependent D-glucose transport. Glucose transport rates, SGLT1 protein, and mRNA expression were all 2-fold higher in the jejunum and 3- to 5-fold higher in the ileum of horses maintained on a hCHO-enriched diet compared to pasture forage. Activity of the disaccharidases was unaltered by diet. In a well-controlled study, we determined SGLT1 expression in the duodenal and ileal biopsies of horses switched, gradually over a 2-month period, from low (<1.0 g/kg bwt/day) to high hCHO (6.0 g/kg bwt/day) diets of known composition. We show that SGLT1 expression is enhanced, with time, 2-fold in the duodenum and 3.3-fold in the ileum. The study has important implications for dietary management of the horse.

Item Type: Article
Authors :
NameEmailORCID
Dyer, JUNSPECIFIEDUNSPECIFIED
Al-Rammahi, MUNSPECIFIEDUNSPECIFIED
Waterfall, LUNSPECIFIEDUNSPECIFIED
Salmon, KSUNSPECIFIEDUNSPECIFIED
Geor, RJUNSPECIFIEDUNSPECIFIED
Bouré, LUNSPECIFIEDUNSPECIFIED
Edwards, GBUNSPECIFIEDUNSPECIFIED
Proudman, CJc.proudman@surrey.ac.ukUNSPECIFIED
Shirazi-Beechey, SPUNSPECIFIEDUNSPECIFIED
Date : June 2009
Identification Number : 10.1007/s00424-008-0620-4
Uncontrolled Keywords : Adaptation, Physiological, Animals, Dietary Carbohydrates, Female, Glucose Transporter Type 2, Horses, Ileum, Immunohistochemistry, Jejunum, Male, Microvilli, RNA, Messenger, Sodium-Glucose Transporter 1, Solubility, Sucrase, alpha-Glucosidases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:57
Last Modified : 17 May 2017 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/825775

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