University of Surrey

Test tubes in the lab Research in the ATI Dance Research

A cluster of basic amino acids in the factor X serine protease mediates surface attachment of adenovirus/FX complexes.

Duffy, MR, Bradshaw, AC, Parker, AL, McVey, JH and Baker, AH (2011) A cluster of basic amino acids in the factor X serine protease mediates surface attachment of adenovirus/FX complexes. J Virol, 85 (20). pp. 10914-10919.

Full text not available from this repository.

Abstract

Hepatocyte transduction following intravenous administration of adenovirus 5 (Ad5) is mediated by interaction between coagulation factor X (FX) and the hexon. The FX serine protease (SP) domain tethers the Ad5/FX complex to hepatocytes through binding heparan sulfate proteoglycans (HSPGs). Here, we identify the critical HSPG-interacting residues of FX. We generated an FX mutant by modifying seven residues in the SP domain. Surface plasmon resonance demonstrated that mutations did not affect binding to Ad5. FX-mediated, HSPG-associated cell binding and transduction were abolished. A cluster of basic amino acids in the SP domain therefore mediates surface interaction of the Ad/FX complex.

Item Type: Article
Authors :
NameEmailORCID
Duffy, MRUNSPECIFIEDUNSPECIFIED
Bradshaw, ACUNSPECIFIEDUNSPECIFIED
Parker, ALUNSPECIFIEDUNSPECIFIED
McVey, JHj.mcvey@surrey.ac.ukUNSPECIFIED
Baker, AHUNSPECIFIEDUNSPECIFIED
Date : October 2011
Identification Number : https://doi.org/10.1128/JVI.05382-11
Uncontrolled Keywords : Adenoviridae, Amino Acid Substitution, Amino Acids, Basic, Factor X, Heparan Sulfate Proteoglycans, Humans, Mutagenesis, Site-Directed, Mutant Proteins, Protein Binding, Surface Plasmon Resonance
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:56
Last Modified : 17 May 2017 14:46
URI: http://epubs.surrey.ac.uk/id/eprint/825745

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800