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Interleukin 2 (IL-2) production by mitogen stimulated bovine peripheral blood lymphocytes and its assay.

Oldham, G and Williams, L (1984) Interleukin 2 (IL-2) production by mitogen stimulated bovine peripheral blood lymphocytes and its assay. Vet Immunol Immunopathol, 7 (3-4). pp. 201-212.

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Abstract

Culture medium from bovine peripheral blood mononuclear cells stimulated with the mitogens phytohaemagglutinin (PHA) or Concanavalin A (Con A) was found to maintain the proliferation of Con A blasts in vitro. The factor responsible for this activity was not absorbable with bovine erythrocytes or fresh peripheral blood lymphocytes but was removed by Con A blasts. Production of this factor was dependent on the dose of mitogen used and was greatest after 24 h culture compared to 48 h. Quantitative determinations of factor activity in supernatants were carried out by regression analysis of logit transformed data from assays measuring the maintenance of Con A blast proliferation by supernatants.

Item Type: Article
Authors :
NameEmailORCID
Oldham, GUNSPECIFIEDUNSPECIFIED
Williams, LUNSPECIFIEDUNSPECIFIED
Date : October 1984
Uncontrolled Keywords : Animals, Cattle, Concanavalin A, In Vitro Techniques, Interleukin-2, Lymphocyte Activation, Phytohemagglutinins, T-Lymphocytes
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:56
Last Modified : 17 May 2017 09:56
URI: http://epubs.surrey.ac.uk/id/eprint/825705

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