University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe.

Anjum, MF, Jones, E, Morrison, V, Tozzoli, R, Morabito, S, Toth, I, Nagy, B, Smith, G, Aspan, A, Nielsen, EM , Fach, P, Herrera-León, S, Woodward, MJ and LA Ragione, RM (2013) Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe. Epidemiol Infect. pp. 1-10.

Full text not available from this repository.

Abstract

SUMMARY The presence of 10 virulence genes was examined using polymerase chain reaction (PCR) in 365 European O157 and non-O157 Escherichia coli isolates associated with verotoxin production. Strain-specific PCR data were analysed using hierarchical clustering. The resulting dendrogram clearly separated O157 from non-O157 strains. The former clustered typical high-risk seropathotype (SPT) A strains from all regions, including Sweden and Spain, which were homogenous by Cramer's V statistic, and strains with less typical O157 features mostly from Hungary. The non-O157 strains divided into a high-risk SPTB harbouring O26, O111 and O103 strains, a group pathogenic to pigs, and a group with few virulence genes other than for verotoxin. The data demonstrate SPT designation and selected PCR separated verotoxigenic E. coli of high and low risk to humans; although more virulence genes or pulsed-field gel electrophoresis will need to be included to separate high-risk strains further for epidemiological tracing.

Item Type: Article
Authors :
NameEmailORCID
Anjum, MFUNSPECIFIEDUNSPECIFIED
Jones, EUNSPECIFIEDUNSPECIFIED
Morrison, VUNSPECIFIEDUNSPECIFIED
Tozzoli, RUNSPECIFIEDUNSPECIFIED
Morabito, SUNSPECIFIEDUNSPECIFIED
Toth, IUNSPECIFIEDUNSPECIFIED
Nagy, BUNSPECIFIEDUNSPECIFIED
Smith, GUNSPECIFIEDUNSPECIFIED
Aspan, AUNSPECIFIEDUNSPECIFIED
Nielsen, EMUNSPECIFIEDUNSPECIFIED
Fach, PUNSPECIFIEDUNSPECIFIED
Herrera-León, SUNSPECIFIEDUNSPECIFIED
Woodward, MJUNSPECIFIEDUNSPECIFIED
LA Ragione, RMr.laragione@surrey.ac.ukUNSPECIFIED
Date : 18 July 2013
Identification Number : 10.1017/S0950268813001635
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:55
Last Modified : 17 May 2017 14:45
URI: http://epubs.surrey.ac.uk/id/eprint/825676

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800