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Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells.

Kaznacheyeva, E, Glushankova, L, Bugaj, V, Zimina, O, Skopin, A, Alexeenko, V, Tsiokas, L, Bezprozvanny, I and Mozhayeva, GN (2007) Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells. J Biol Chem, 282 (32). pp. 23655-23662.

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Abstract

In most non-excitable cells, calcium (Ca(2+)) release from the inositol 1,4,5-trisphosphate (InsP(3))-sensitive intracellular Ca(2+) stores is coupled to Ca(2+) influx through the plasma membrane Ca(2+) channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca(2+) influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.

Item Type: Article
Authors :
NameEmailORCID
Kaznacheyeva, EUNSPECIFIEDUNSPECIFIED
Glushankova, LUNSPECIFIEDUNSPECIFIED
Bugaj, VUNSPECIFIEDUNSPECIFIED
Zimina, OUNSPECIFIEDUNSPECIFIED
Skopin, AUNSPECIFIEDUNSPECIFIED
Alexeenko, Vv.alexeenko@surrey.ac.ukUNSPECIFIED
Tsiokas, LUNSPECIFIEDUNSPECIFIED
Bezprozvanny, IUNSPECIFIEDUNSPECIFIED
Mozhayeva, GNUNSPECIFIEDUNSPECIFIED
Date : 10 August 2007
Identification Number : 10.1074/jbc.M608378200
Uncontrolled Keywords : Calcium, Calcium Channels, Calcium Signaling, Cell Line, Tumor, Cytosol, Gene Expression Regulation, Neoplastic, Humans, Inositol 1,4,5-Trisphosphate Receptors, Models, Biological, RNA Interference, RNA, Small Interfering, TRPC Cation Channels
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:55
Last Modified : 17 May 2017 09:55
URI: http://epubs.surrey.ac.uk/id/eprint/825668

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