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Infection and transmission dynamics of rKSHV.219 in primary endothelial cells.

Jeffery, HC, Wheat, RL, Blackbourn, DJ, Nash, GB and Butler, LM (2013) Infection and transmission dynamics of rKSHV.219 in primary endothelial cells. J Virol Methods, 193 (1). pp. 251-259.

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Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O'Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.

Item Type: Article
Authors :
NameEmailORCID
Jeffery, HCUNSPECIFIEDUNSPECIFIED
Wheat, RLUNSPECIFIEDUNSPECIFIED
Blackbourn, DJd.blackbourn@surrey.ac.ukUNSPECIFIED
Nash, GBUNSPECIFIEDUNSPECIFIED
Butler, LMUNSPECIFIEDUNSPECIFIED
Date : October 2013
Identification Number : https://doi.org/10.1016/j.jviromet.2013.06.001
Uncontrolled Keywords : Endothelial cell, Kaposi's sarcoma-associated herpes virus, Rkshv.219
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:53
Last Modified : 17 May 2017 14:45
URI: http://epubs.surrey.ac.uk/id/eprint/825546

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