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A Vibrio parahaemolyticus T3SS Effector Mediates Pathogenesis by Independently Enabling Intestinal Colonization and Inhibiting TAK1 Activation.

Zhou, X, Gewurz, BE, Ritchie, JM, Takasaki, K, Greenfeld, H, Kieff, E, Davis, BM and Waldor, MK (2013) A Vibrio parahaemolyticus T3SS Effector Mediates Pathogenesis by Independently Enabling Intestinal Colonization and Inhibiting TAK1 Activation. Cell Rep, 3 (5). pp. 1690-1702.

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Abstract

Vibrio parahaemolyticus type III secretion system 2 (T3SS2) is essential for the organism's virulence, but the effectors required for intestinal colonization and induction of diarrhea by this pathogen have not been identified. Here, we identify a type III secretion system (T3SS2)-secreted effector, VopZ, that is essential for V. parahaemolyticus pathogenicity. VopZ plays distinct, genetically separable roles in enabling intestinal colonization and diarrheagenesis. Truncation of VopZ prevents V. parahaemolyticus colonization, whereas deletion of VopZ amino acids 38-62 abrogates V. parahaemolyticus-induced diarrhea and intestinal pathology but does not impair colonization. VopZ inhibits activation of the kinase TAK1 and thereby prevents the activation of MAPK and NF-κB signaling pathways, which lie downstream. In contrast, the VopZ internal deletion mutant cannot counter the activation of pathways regulated by TAK1. Collectively, our findings suggest that VopZ's inhibition of TAK1 is critical for V. parahaemolyticus to induce diarrhea and intestinal pathology.

Item Type: Article
Authors :
NameEmailORCID
Zhou, XUNSPECIFIEDUNSPECIFIED
Gewurz, BEUNSPECIFIEDUNSPECIFIED
Ritchie, JMj.ritchie@surrey.ac.ukUNSPECIFIED
Takasaki, KUNSPECIFIEDUNSPECIFIED
Greenfeld, HUNSPECIFIEDUNSPECIFIED
Kieff, EUNSPECIFIEDUNSPECIFIED
Davis, BMUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Date : 30 May 2013
Identification Number : https://doi.org/10.1016/j.celrep.2013.03.039
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:52
Last Modified : 17 May 2017 14:45
URI: http://epubs.surrey.ac.uk/id/eprint/825481

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