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Endocytic tubules regulated by Rab GTPases 5 and 11 are used for envelopment of herpes simplex virus.

Hollinshead, M, Johns, HL, Sayers, CL, Gonzalez-Lopez, C, Smith, GL and Elliott, G (2012) Endocytic tubules regulated by Rab GTPases 5 and 11 are used for envelopment of herpes simplex virus. EMBO J, 31 (21). pp. 4204-4220.

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Abstract

Enveloped viruses employ diverse and complex strategies for wrapping at cellular membranes, many of which are poorly understood. Here, an ultrastructural study of herpes simplex virus 1 (HSV1)-infected cells revealed envelopment in tubular membranes. These tubules were labelled by the fluid phase marker horseradish peroxidase (HRP), and were observed to wrap capsids as early as 2 min after HRP addition, indicating that the envelope had recently cycled from the cell surface. Consistent with this, capsids did not colocalise with either the trans-Golgi network marker TGN46 or late endosomal markers, but showed coincidence with the transferrin receptor. Virus glycoproteins were retrieved from the plasma membrane (PM) to label wrapping capsids, a process that was dependent on both dynamin and Rab5. Combined depletion of Rab5 and Rab11 reduced virus yield to <1%, resulting in aberrant localisation of capsids. These results suggest that endocytosis from the PM into endocytic tubules provides the main source of membrane for HSV1, and reveal a new mechanism for virus exploitation of the endocytic pathway.

Item Type: Article
Authors :
NameEmailORCID
Hollinshead, MUNSPECIFIEDUNSPECIFIED
Johns, HLUNSPECIFIEDUNSPECIFIED
Sayers, CLUNSPECIFIEDUNSPECIFIED
Gonzalez-Lopez, CUNSPECIFIEDUNSPECIFIED
Smith, GLUNSPECIFIEDUNSPECIFIED
Elliott, Gg.elliott@surrey.ac.ukUNSPECIFIED
Date : 5 November 2012
Identification Number : https://doi.org/10.1038/emboj.2012.262
Uncontrolled Keywords : Animals, Blotting, Western, Capsid, Cell Membrane, Cells, Cultured, Cercopithecus aethiops, Endocytosis, Fibroblasts, Flow Cytometry, Fluorescent Antibody Technique, Glycoproteins, HeLa Cells, Herpes Simplex, Herpesvirus 1, Human, Horseradish Peroxidase, Humans, Intracellular Membranes, Lysosome-Associated Membrane Glycoproteins, Vero Cells, Viral Envelope Proteins, Virus Assembly, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, trans-Golgi Network
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:51
Last Modified : 17 May 2017 14:45
URI: http://epubs.surrey.ac.uk/id/eprint/825389

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