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The development of a single-step solid-phase immuno-extraction for morphine

Rashid, BA, Kwasowski, P and Stevenson, D (1996) The development of a single-step solid-phase immuno-extraction for morphine Pharmaceutical Sciences, 2 (2). pp. 115-116.

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If suitable adsorbents can be developed, solid-phase extraction using antibodies to selected analytes is potentially much more selective than liquid-liquid or solid-phase extraction. This preliminary study uses antisera to morphine immobilized on controlled-pore glass to investigate the feasibility of developing a selective extraction procedure. Columns are washed with 0.3% HCl and primed with phosphate-buffered saline. Phosphate-buffered saline of different pH and with different organic modifiers was investigated as elution solvent. The results indicate that morphine could be extracted from aqueous solution using an immune-extraction column and desorbed in a 1-mL fraction using 60% ethanol in phosphate-buffered saline of pH 2.

Item Type: Article
Authors :
Date : 1 December 1996
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:51
Last Modified : 17 May 2017 14:45

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