University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Selective extraction via immunoaffinity sorbents and molecular imprinted polymers

Stevenson, D (1998) Selective extraction via immunoaffinity sorbents and molecular imprinted polymers Journal de Pharmacie de Belgique, 53 (3).

Full text not available from this repository.


The analysis of trace organics, such as drugs, metabolites and biomarkers of disease in biological fluids remains a challenging task. Modern methods may require lower detection limits, higher sample throughput, analytes that are difficult to extract, analysis outside the laboratory or measurement of unstable analytes. For many analytes sample preparation remains the rate limiting step and the largest contributor to analytical error. This paper will demonstrate the feasibility of developing highly selective sorbents for solid phase extraction. We have successfully immobilised antibodies to the pesticides chlortoluron and isoproturon and demonstrated their ability to selectively extract these phenylureas from environmental water samples (1,2). This work has been extended to include the successful immobilisation of antibodies to the drugs morphine and clenbuterol (3-5). Selective extraction from plasma and urine can be accomplished using aqueous washing and elution and elution solvents. A generalised protocol involves loading the sample, washing the immunosorbent with phosphate buffered saline (pH 7) and then eluting the analyte in 1 ml of phosphate buffered saline (pH 2-4) containing 40-50 % methanol or ethanol. Samples were subsequently analysed by HPLC with electrochemical detection for morphine and HPLC-UV for clenbuterol. Recently the use of molecular imprinted polymers as antibody mimics has been described. Preliminary work to investigate the feasibility of using these as selective solid phases for the drugs tamoxifen(6) and atenolol will be described.

Item Type: Article
Divisions : Surrey research (other units)
Authors :
Date : 1 December 1998
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:51
Last Modified : 24 Jan 2020 17:48

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800