In vitro cytotoxic activity of Aesculus indica against breast adenocarcinoma cell line (MCF-7) and phytochemical analysis.

Bibi, Y, Nisa, S, Zia, M, Waheed, A, Ahmed, S and Chaudhary, MF (2012) In vitro cytotoxic activity of Aesculus indica against breast adenocarcinoma cell line (MCF-7) and phytochemical analysis. Pak J Pharm Sci, 25 (1). pp. 183-187.

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Abstract

Aesculus indica (Linn.) (Sapindaceae) is an ethanobotanically important plant specie traditionally used against rheumatism, skin and vein complaints. Cytotoxic potential of Aesculus indica crude leaf extract and its fractions was investigated against MCF-7 cell line. Crude extract of Aesculus indica was prepared in methanol by maceration technique. Crude extract was fractionated into four organic and one aqueous fraction on polarity basis. MTT assay was used to evaluate the reduction of viability of MCF-7 breast cancer cell line. Cell viability was inhibited by Aesculus indica crude extract in a dose dependent manner ranging from 34.2% at 10 μg/ml to 94% at 500μg/ml. Activity was found in an ascending order from hexane showing 29.8% inhibition to aqueous fraction indicating maximum inhibition, 60%. Phytochemical analysis of crude and fractionated extracts revealed presence of flavonoids, saponins, coumarins and tannins upto varying degrees. Methanol and aqueous fraction of methanol extract of Aesculus indica can be good source of cytotoxic compounds.

Item Type:	Article
Authors :	Name Email ORCID Bibi, Y UNSPECIFIED UNSPECIFIED Nisa, S UNSPECIFIED UNSPECIFIED Zia, M UNSPECIFIED UNSPECIFIED Waheed, A a.waheed@surrey.ac.uk UNSPECIFIED Ahmed, S UNSPECIFIED UNSPECIFIED Chaudhary, MF UNSPECIFIED UNSPECIFIED
Date :	January 2012
Uncontrolled Keywords :	Adenocarcinoma, Aesculus, Breast Neoplasms, Cell Line, Tumor, Cell Survival, Chemical Fractionation, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Female, Humans, Plant Extracts, Plant Leaves
Depositing User :	Symplectic Elements
Date Deposited :	17 May 2017 09:47
Last Modified :	17 May 2017 14:44
URI:	http://epubs.surrey.ac.uk/id/eprint/825146

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