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Escherichia coli O115 forms fewer attaching and effacing lesions in the ovine colon in the presence of E. coli O157:H7.

La Ragione, RM, Best, A, Aktan, I, Clifton-Hadley, FA, Dugdale, AH, Weyer, U, Crawley, T, Townsend, P, Cooley, WA, Johnson, L , Wangoo, A, Woodward, MJ and Wales, AD (2012) Escherichia coli O115 forms fewer attaching and effacing lesions in the ovine colon in the presence of E. coli O157:H7. Research in Veterinary Science.

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Abstract

Escherichia coli O115 has been isolated from healthy sheep and was shown to be associated with attaching-effacing (AE) lesions in the large intestine. Following previous observations of interactions between E. coli O157 and O26, the aim of the present study was to assess what influence an O115 AE E. coli (AEEC) would have on E. coli O157 colonisation in vitro and in vivo. We report that E. coli O115- and O157-associated AE lesions were observed on HEp-2 cells and on the mucosa of ligated ovine spiral colon. In single strain inoculum, E. coli O115 associated intimately with HEp-2 cells and the spiral colon in greater numbers than E. coli O157:H7. However, in mixed inoculum studies, the number of E. coli O115 AE lesions was significantly reduced suggesting negative interference by E. coli O157. Use of the ligated colon model in the present work has allowed in vitro observations to be extended and confirmed whilst using a minimum of experimental animals. The findings support a hypothesis that some AEEC can inhibit adhesion of other AEEC in vivo. The mechanisms involved may prove to be of utility in the control of AE pathovars.

Item Type: Article
Authors :
NameEmailORCID
La Ragione, RMr.laragione@surrey.ac.ukUNSPECIFIED
Best, AUNSPECIFIEDUNSPECIFIED
Aktan, Ii.aktan@surrey.ac.ukUNSPECIFIED
Clifton-Hadley, FAUNSPECIFIEDUNSPECIFIED
Dugdale, AHUNSPECIFIEDUNSPECIFIED
Weyer, UUNSPECIFIEDUNSPECIFIED
Crawley, TUNSPECIFIEDUNSPECIFIED
Townsend, PUNSPECIFIEDUNSPECIFIED
Cooley, WAUNSPECIFIEDUNSPECIFIED
Johnson, LUNSPECIFIEDUNSPECIFIED
Wangoo, AUNSPECIFIEDUNSPECIFIED
Woodward, MJm.woodward@surrey.ac.ukUNSPECIFIED
Wales, ADUNSPECIFIEDUNSPECIFIED
Date : 29 August 2012
Identification Number : 10.1016/j.rvsc.2011.07.026
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:43
Last Modified : 17 May 2017 14:44
URI: http://epubs.surrey.ac.uk/id/eprint/824877

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