University of Surrey

Test tubes in the lab Research in the ATI Dance Research

RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): Genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication.

Anthony, SJ, Darpel, KE, Belaganahalli, MN, Maan, N, Nomikou, K, Sutton, G, Attoui, H, Maan, S and Mertens, PP (2011) RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): Genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication. Virology, 420 (2). pp. 164-171.

Full text not available from this repository.

Abstract

This paper reports a concatemeric RNA in a strain of epizootic haemorrhagic disease virus (EHDV) serotype 5. Sequencing showed that the concatemeric RNA contains two identical full-length copies of genome segment 9, arranged in series, which has apparently replaced the monomeric form of the segment. In vitro translation demonstrated that the concatemeric RNA can act as a viable template for VP6 translation, but that no double-sized protein is produced. Studies were also performed to assess whether mutations might be easily introduced into the second copy (which might indicate some potential evolutionary significance of a concatemeric RNA segment), however multiple (n=40) passages generated no changes in the sequence of either the upstream or downstream segments. Further, we present results that demonstrate the presence of concatemers or partial gene duplications in multiple segments of different orbiviruses (in tissue culture and purified virus), suggesting their generation is likely to be a normal feature of orbivirus replication.

Item Type: Article
Authors :
NameEmailORCID
Anthony, SJUNSPECIFIEDUNSPECIFIED
Darpel, KEk.darpel@surrey.ac.ukUNSPECIFIED
Belaganahalli, MNUNSPECIFIEDUNSPECIFIED
Maan, NUNSPECIFIEDUNSPECIFIED
Nomikou, KUNSPECIFIEDUNSPECIFIED
Sutton, GUNSPECIFIEDUNSPECIFIED
Attoui, HUNSPECIFIEDUNSPECIFIED
Maan, SUNSPECIFIEDUNSPECIFIED
Mertens, PPUNSPECIFIEDUNSPECIFIED
Date : 25 November 2011
Identification Number : 10.1016/j.virol.2011.09.009
Uncontrolled Keywords : Animals, Australia, Base Sequence, Cell Line, Cricetinae, Genes, Viral, Genetic Variation, Genome, Viral, Hemorrhagic Disease Virus, Epizootic, Nucleic Acid Conformation, Phylogeny, RNA, Viral, Sequence Analysis, RNA, Virus Replication
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:43
Last Modified : 17 May 2017 14:44
URI: http://epubs.surrey.ac.uk/id/eprint/824869

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800