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Characterization of the Temporal Induction of Hepatic Xenobiotic-Metabolizing Enzymes by Glucosinolates and Isothiocyanates: Requirement for at Least a 6 h Exposure To Elicit Complete Induction Profile.

Abdull Razis, AF, Bagatta, M, De Nicola, GR, Iori, R, Plant, N and Ioannides, C (2012) Characterization of the Temporal Induction of Hepatic Xenobiotic-Metabolizing Enzymes by Glucosinolates and Isothiocyanates: Requirement for at Least a 6 h Exposure To Elicit Complete Induction Profile. J Agric Food Chem, 60 (22). pp. 5556-5564.

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Abstract

A mechanism of action of chemopreventive glucosinolates/isothiocyanates, established largely in vitro, is to modulate carcinogen-metabolizing enzymes. Extrapolation in vivo involves relating in vitro concentrations to plasma/tissue concentrations attained in vivo, thus assuming that even transient exposure modulates enzyme activity. To test this hypothesis, precision-cut rat liver slices were incubated with glucosinolates for up to 24 h, and the O-dealkylation of methoxyresorufin and ethoxyresorufin was determined; increased activities were observed only at incubations of at least 6 h. To evaluate phase II enzymes, isothiocyanates, namely, sulforaphane, erucin, and phenethyl isothiocyanate, were similarly incubated; quinone reductase increased after incubation for 6 h or longer. When glutathione S-transferase was monitored, the phenethyl isothiocyanate-manifested rise necessitated at least a 6 h incubation, whereas in the case of sulforaphane and erucin, the activity was elevated after only 2 h. It is inferred that a rise in carcinogen-metabolizing enzymes by glucosinolates/isothiocyanates necessitates tissue exposure of at least 6 h.

Item Type: Article
Authors :
NameEmailORCID
Abdull Razis, AFUNSPECIFIEDUNSPECIFIED
Bagatta, MUNSPECIFIEDUNSPECIFIED
De Nicola, GRUNSPECIFIEDUNSPECIFIED
Iori, RUNSPECIFIEDUNSPECIFIED
Plant, Nn.plant@surrey.ac.ukUNSPECIFIED
Ioannides, Cc.ioannides@surrey.ac.ukUNSPECIFIED
Date : 6 June 2012
Identification Number : 10.1021/jf3011195
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:43
Last Modified : 17 May 2017 14:44
URI: http://epubs.surrey.ac.uk/id/eprint/824856

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