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Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma

Pulido, J, Kottke, T, Thompson, J, Galivo, F, Wongthida, P, Diaz, RM, Rommelfanger, D, Ilett, E, Pease, L, Pandha, H , Harrington, K, Selby, P, Melcher, A and Vile, R (2012) Using virally expressed melanoma cDNA libraries to identify tumor-associated antigens that cure melanoma Nature Biotechnology, 30 (4). pp. 337-343.

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Abstract

Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumor-specific CD4 + interleukin (IL)-17 recall response in vitro. We used this characteristic IL-17 response to screen the VSV-cDNA library and identified three different VSV-cDNA virus clones that, when used in combination but not alone, achieved the same efficacy against tumors as the complete parental virus library. VSV-expressed cDNA libraries can therefore be used to identify tumor rejection antigens that can cooperate to induce anti-tumor responses. This technology should be applicable to antigen discovery for other cancers, as well as for other diseases in which immune reactivity against more than one target antigen contributes to disease pathology. © 2012 Nature America, Inc. All rights reserved.

Item Type: Article
Authors :
NameEmailORCID
Pulido, JUNSPECIFIEDUNSPECIFIED
Kottke, TUNSPECIFIEDUNSPECIFIED
Thompson, JUNSPECIFIEDUNSPECIFIED
Galivo, FUNSPECIFIEDUNSPECIFIED
Wongthida, PUNSPECIFIEDUNSPECIFIED
Diaz, RMUNSPECIFIEDUNSPECIFIED
Rommelfanger, DUNSPECIFIEDUNSPECIFIED
Ilett, EUNSPECIFIEDUNSPECIFIED
Pease, LUNSPECIFIEDUNSPECIFIED
Pandha, Hh.pandha@surrey.ac.ukUNSPECIFIED
Harrington, KUNSPECIFIEDUNSPECIFIED
Selby, PUNSPECIFIEDUNSPECIFIED
Melcher, AUNSPECIFIEDUNSPECIFIED
Vile, RUNSPECIFIEDUNSPECIFIED
Date : 1 April 2012
Identification Number : 10.1038/nbt.2157
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:43
Last Modified : 17 May 2017 14:44
URI: http://epubs.surrey.ac.uk/id/eprint/824827

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