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The effect of pH on the block by L-cis-diltiazem and amiloride of the cyclic GMP-activated conductance of salamander rods.

McLatchie, LM and Matthews, HR (1994) The effect of pH on the block by L-cis-diltiazem and amiloride of the cyclic GMP-activated conductance of salamander rods. Proc Biol Sci, 255 (1344). pp. 231-236.

Full text not available from this repository.

Abstract

Block by L-cis-diltiazem and amiloride of the cyclic GMP-activated conductance was studied in inside-out patches excised from the salamander rod outer segment. When cytoplasmic pH was varied, the steady-state level of block by L-cis-diltiazem changed in the way that would be predicted if it were the protonated ammonium group that is responsible for effecting block. This is in contrast to the recent results of Haynes (J. gen. Physiol. 100, 783 (1992)) in catfish cones, where no such change was seen. Amiloride was found to block the conductance with a similar voltage dependence to that of L-cis-diltiazem. The dependence of amiloride block on cytoplasmic pH was found to be shifted relative to that of L-cis-diltiazem, consistent with the 1 pH-unit higher pKa value of amiloride and the idea that it is only the charged form of amiloride which can effect block. This suggests that the results seen with L-cis-diltiazem were indeed due to changes in the proportion of blocker in the protonated form, and not to effects of protons on the channel.

Item Type: Article
Authors :
NameEmailORCID
McLatchie, LMl.mclatchie@surrey.ac.ukUNSPECIFIED
Matthews, HRUNSPECIFIEDUNSPECIFIED
Date : 22 March 1994
Identification Number : https://doi.org/10.1098/rspb.1994.0033
Uncontrolled Keywords : Ambystoma, Amiloride, Animals, Cyclic GMP, Cytoplasm, Diltiazem, Electric Conductivity, Hydrogen-Ion Concentration, In Vitro Techniques, Kinetics, Membrane Potentials, Retinal Rod Photoreceptor Cells, Rod Cell Outer Segment, Time Factors
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:40
Last Modified : 17 May 2017 09:40
URI: http://epubs.surrey.ac.uk/id/eprint/824649

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