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Identification of a novel prophage regulator in Escherichia coli controlling the expression of type III secretion.

Flockhart, AF, Tree, JJ, Xu, X, Karpiyevich, M, McAteer, SP, Rosenblum, R, Shaw, DJ, Low, CJ, Best, A, Gannon, V , Laing, C, Murphy, KC, Leong, JM, Schneiders, T, La Ragione, R and Gally, DL (2012) Identification of a novel prophage regulator in Escherichia coli controlling the expression of type III secretion. Mol Microbiol, 83 (1). pp. 208-223.

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Abstract

This study has identified horizontally acquired genomic regions of enterohaemorrhagic Escherichia coli O157:H7 that regulate expression of the type III secretion (T3S) system encoded by the locus of enterocyte effacement (LEE). Deletion of O-island 51, a 14.93 kb cryptic prophage (CP-933C), resulted in a reduction in LEE expression and T3S. The deletion also had a reduced capacity to attach to epithelial cells and significantly reduced E. coli O157 excretion levels from sheep. Further characterization of O-island 51 identified a novel positive regulator of the LEE, encoded by ecs1581 in the E. coli O157:H7 strain Sakai genome and present but not annotated in the E. coli strain EDL933 sequence. Functionally important residues of ECs1581 were identified based on phenotypic variants present in sequenced E. coli strains and the regulator was termed RgdR based on a motif demonstrated to be important for stimulation of gene expression. While RgdR activated expression from the LEE1 promoter in the presence or absence of the LEE-encoded regulator (Ler), RgdR stimulation of T3S required ler and Ler autoregulation. RgdR also controlled the expression of other phenotypes, including motility, indicating that this new family of regulators may have a more global role in E. coli gene expression.

Item Type: Article
Authors :
NameEmailORCID
Flockhart, AFUNSPECIFIEDUNSPECIFIED
Tree, JJUNSPECIFIEDUNSPECIFIED
Xu, XUNSPECIFIEDUNSPECIFIED
Karpiyevich, MUNSPECIFIEDUNSPECIFIED
McAteer, SPUNSPECIFIEDUNSPECIFIED
Rosenblum, RUNSPECIFIEDUNSPECIFIED
Shaw, DJUNSPECIFIEDUNSPECIFIED
Low, CJUNSPECIFIEDUNSPECIFIED
Best, AUNSPECIFIEDUNSPECIFIED
Gannon, VUNSPECIFIEDUNSPECIFIED
Laing, CUNSPECIFIEDUNSPECIFIED
Murphy, KCUNSPECIFIEDUNSPECIFIED
Leong, JMUNSPECIFIEDUNSPECIFIED
Schneiders, TUNSPECIFIEDUNSPECIFIED
La Ragione, Rr.laragione@surrey.ac.ukUNSPECIFIED
Gally, DLUNSPECIFIEDUNSPECIFIED
Date : January 2012
Identification Number : 10.1111/j.1365-2958.2011.07927.x
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:38
Last Modified : 17 May 2017 14:43
URI: http://epubs.surrey.ac.uk/id/eprint/824506

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