University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Microarray based comparative genotyping of gentamicin resistant Escherichia coli strains from food animals and humans.

Szmolka, A, Anjum, MF, La Ragione, RM, Kaszanyitzky, EJ and Nagy, B (2011) Microarray based comparative genotyping of gentamicin resistant Escherichia coli strains from food animals and humans. Vet Microbiol.

Full text not available from this repository.

Abstract

Recent data from the European and Hungarian Antimicrobial Resistance Monitoring Systems have indicated that the routine use of gentamicin in human and veterinary medicine frequently leads to the selection of gentamicin resistance in Escherichia coli. The aim of this study was to provide molecular characterization of gentamicin resistance in clinical and commensal E. coli strains representing humans and food producing animals by genotyping for antimicrobial resistance and virulence using a miniaturized microarray. All 50 strains tested proved to be multidrug resistant defined as resistance to three or more antimicrobial classes. Antimicrobial resistances genes such as aadA1-like, strB, bla(TEM), sul1 and tet(A) or tet(B), and corresponding phenotypes (streptomycin-, ampicillin-, sulfamethoxazole- and tetracycline resistance) were detected in >50% of isolates regardless of the host or clinical background. However, certain genes encoding gentamicin resistance such as aac(6')-Ib and ant(2″)-Ia as well as catB3-like genes for phenicol resistance were only detected in human isolates. Among virulence genes, the increased serum survival gene iss was predominant in all host groups. Although the majority of gentamicin resistant E. coli strains were characterized by diverse antimicrobial resistance, and virulence gene patterns, accentuated links between catB3-like, aac(6')-Ib, bla(CTX-M-1) and sat genes could be detected in human strains. Further resistance/virulence gene associations (tet(A) with iroN and iss) were detected in poultry strains. In conclusion, the simultaneous characterization of antimicrobial resistance and virulence genotypes of representative clinical and commensal strains of E. coli should be useful for the identification of emerging genotypes with human and or animal health implications.

Item Type: Article
Authors :
NameEmailORCID
Szmolka, AUNSPECIFIEDUNSPECIFIED
Anjum, MFUNSPECIFIEDUNSPECIFIED
La Ragione, RMr.laragione@surrey.ac.ukUNSPECIFIED
Kaszanyitzky, EJUNSPECIFIEDUNSPECIFIED
Nagy, BUNSPECIFIEDUNSPECIFIED
Date : 2 October 2011
Identification Number : 10.1016/j.vetmic.2011.09.030
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:37
Last Modified : 17 May 2017 14:43
URI: http://epubs.surrey.ac.uk/id/eprint/824460

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800