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Quantitative measurement of 3-O-methyl-D-glucose by gas chromatography-mass spectrometry as a measure of glucose transport in vivo.

Shojaee-Moradie, F, Jackson, NC, Jones, RH, Mallet, AI, Hovorka, R and Umpleby, AM (1996) Quantitative measurement of 3-O-methyl-D-glucose by gas chromatography-mass spectrometry as a measure of glucose transport in vivo. J Mass Spectrom, 31 (9). pp. 961-966.

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Abstract

Existing methods of measuring glucose kinetics are subject to errors. There is considerable interest in improved methods of measuring glucose kinetics to allow the development of new regimes for the treatment of diabetes mellitus. 3-O-Methyl-D-glucose is transported but not metabolized and therefore allows independent estimation of transport parameters. We describe a method by which 3-O-methyl-D-glucose in plasma samples can be measured in protocols during which glucose flux is assessed with simultaneous use of two isotopically labeled glucoses to quantitate and validate measurements of the rate of glucose appearance and disappearance. Quantitative gas chromatographic/mass spectrometric (GC/MS) analysis of 3-O-methyl-D-glucose, D-glucose, D-[U-13C] glucose and D-[6,6-2H2] glucose in human plasma using methoxime-trimethylsilyl ether derivatives is described. Measurements of all four derivatives were performed together in a small sample volume (50 microliters) with high precision. The intra-assay (inter-assay) coefficients of variation at an isotope content of 0.25 atom% excess for D-[6,6-2H2] glucose, D-[U-13C] glucose and 3-O-methyl-D-glucose were 0.8% (1.0%), 0.5% (4.0%) and 0.1% (3.7%), respectively. This method provides the basis for quantitative estimation of parameters of glucose kinetics in man and the rates of glucose flux across the cell membrane.

Item Type: Article
Authors :
NameEmailORCID
Shojaee-Moradie, FUNSPECIFIEDUNSPECIFIED
Jackson, NCn.jackson@surrey.ac.ukUNSPECIFIED
Jones, RHUNSPECIFIEDUNSPECIFIED
Mallet, AIUNSPECIFIEDUNSPECIFIED
Hovorka, RUNSPECIFIEDUNSPECIFIED
Umpleby, AMUNSPECIFIEDUNSPECIFIED
Date : September 1996
Identification Number : 10.1002/(SICI)1096-9888(199609)31:9<961::AID-JMS359>3.0.CO;2-H
Uncontrolled Keywords : 3-O-Methylglucose, Biological Transport, Active, Calibration, Gas Chromatography-Mass Spectrometry, Glucose, Humans, Trimethylsilyl Compounds
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:32
Last Modified : 17 May 2017 14:43
URI: http://epubs.surrey.ac.uk/id/eprint/824113

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