University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Effect of corticosterone on protein degradation in isolated rat soleus and extensor digitorum longus muscles.

Bowes, SB, Jackson, NC, Papachristodoulou, D, Umpleby, AM and Sönksen, PH (1996) Effect of corticosterone on protein degradation in isolated rat soleus and extensor digitorum longus muscles. J Endocrinol, 148 (3). pp. 501-507.

Full text not available from this repository.

Abstract

The net catabolic effect of glucocorticoids on protein metabolism is well documented but the acute and chronic effect of glucocorticoids on protein breakdown remains controversial. In the present studies protein breakdown was measured by the release of tyrosine from the isolated soleus and extensor digitorum longus (EDL) muscles of control rats and rats treated with corticosterone (10 mg/100 g body weight/day) for 5 days. The effect of corticosterone in arresting growth was confirmed since corticosterone-treated rats weighed significantly less than control rats after 2, 3, 4 and 5 days of treatment (P < 0.001). Furthermore, the weights of soleus and EDL muscles from corticosterone-treated rats were significantly reduced (P < 0.001, at least P < 0.05 respectively) compared with muscles from control rats on days 3-5. In the EDL muscle tyrosine release was significantly elevated after corticosterone treatment for 2 days (257 +/- 21 nmol/g tissue/h, P < 0.05), 3 days (205 +/- 9 nmol/g tissue/h, P < 0.01), 4 days (255 +/- 20 nmol/g tissue/h, P < 0.005) and 5 days (218 +/- 8 nmol/g tissue/h, P < 0.05) compared with EDL from control rats (192 +/- 13, 171 +/- 7, 187 +/- 7, 180 +/- 12 nmol/g tissue/h respectively). In the soleus muscle, tyrosine release was significantly elevated after corticosterone treatment for 2 days (226 +/- 14 nmol/g tissue/h, P < 0.001), 3 days (223 +/- 16 nmol/g tissue/h, P < 0.001) and 4 days (199 +/- 10 nmol/g tissue/h, P < 0.001) compared with control rats (158 +/- 7, 132 +/- 6 and 153 +/- 7 nmol/g tissue/h respectively). After 5 days there was no significant difference in tyrosine release from soleus muscle between corticosterone-treated (176 +/- 15 nmol/g tissue/h) and control rats (157 +/- 6 nmol/g tissue/h). Plasma glucose concentrations were not significantly different in rats treated with corticosterone and control rats whilst insulin levels were significantly raised in the corticosterone-treated rats on all days compared with control rats (P < 0.05 on day 1; P < 0.001 on days 2, 3, 4 and 5). It is suggested that insulin may have prevented hyperglycaemia developing in the corticosterone-treated rats. Results from these studies indicate that the acute effect of glucocorticoids is to increase muscle proteolysis but this is not maintained with longer-term treatment.

Item Type: Article
Authors :
NameEmailORCID
Bowes, SBUNSPECIFIEDUNSPECIFIED
Jackson, NCn.jackson@surrey.ac.ukUNSPECIFIED
Papachristodoulou, DUNSPECIFIEDUNSPECIFIED
Umpleby, AMUNSPECIFIEDUNSPECIFIED
Sönksen, PHUNSPECIFIEDUNSPECIFIED
Date : March 1996
Uncontrolled Keywords : Animals, Blood Glucose, Corticosterone, Culture Techniques, Insulin, Male, Muscle Proteins, Muscle, Skeletal, Rats, Rats, Sprague-Dawley, Rats, Wistar, Tyrosine
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:32
Last Modified : 17 May 2017 14:43
URI: http://epubs.surrey.ac.uk/id/eprint/824112

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800