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Mono-, di- and triacylglycerol concentrations in human plasma: effects of heparin injection and of a high-fat meal.

Fielding, BA, Humphreys, SM, Allman, RF and Frayn, KN (1993) Mono-, di- and triacylglycerol concentrations in human plasma: effects of heparin injection and of a high-fat meal. Clin Chim Acta, 216 (1-2). pp. 167-173.

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Abstract

A sensitive method has been developed to measure specific mono-, di- and triacylglycerol concentrations in human plasma, using thin-layer chromatography and enzymatic assay. The levels of partial acylglycerols in human plasma from fasting subjects were lower than previous reports had suggested and amounted to less than 3% of the total acylglycerols. After heparin injection the plasma monoacylglycerol concentration increased markedly (P < 0.01) while the triacylglycerol concentration decreased significantly (P < 0.001). The plasma diacylglycerol concentration did not change significantly although it increased as a percentage of the total (P < 0.05); after heparin partial acylglycerols accounted for more than 10% of the total. After a high-fat meal the plasma concentrations of di- and triacylglycerol increased approximately two-fold (P < 0.005) but no significant change was observed for mono-acylglycerol. The percentage contribution of partial acylglycerols was unchanged (2.6% fasting, 2.4% postprandially).

Item Type: Article
Authors :
NameEmailORCID
Fielding, BAb.fielding@surrey.ac.ukUNSPECIFIED
Humphreys, SMUNSPECIFIEDUNSPECIFIED
Allman, RFUNSPECIFIEDUNSPECIFIED
Frayn, KNUNSPECIFIEDUNSPECIFIED
Date : 16 July 1993
Uncontrolled Keywords : Adult, Dietary Carbohydrates, Dietary Fats, Diglycerides, Fatty Acids, Nonesterified, Female, Glycerol, Heparin, Humans, Male, Triglycerides
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:29
Last Modified : 17 May 2017 14:43
URI: http://epubs.surrey.ac.uk/id/eprint/823938

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