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Sodium nitroprusside-induced rat fundus relaxation is ryanodine-sensitive and involves L-type Ca2+ channel and small conductance Ca(2+)-sensitive K+ channel components.

Geeson, J, Larsson, K, Hourani, SM and Toms, NJ (2002) Sodium nitroprusside-induced rat fundus relaxation is ryanodine-sensitive and involves L-type Ca2+ channel and small conductance Ca(2+)-sensitive K+ channel components. Auton Autacoid Pharmacol, 22 (5-6). pp. 297-301.

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Abstract

1 The aim of this study was to examine whether sodium nitroprusside (SNP)-induced relaxation of rat fundus longitudinal smooth muscle involves ryanodine-sensitive Ca2+ release. 2 SNP (300 nM-30 microM) elicited concentration-dependent relaxation of precontracted (1 microM carbachol) rat fundus, an effect almost abolished by the selective guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one (ODQ, 10 microM). 3 SNP-mediated relaxations were almost abolished by 10 microM ryanodine. 4 SNP-mediated relaxations were also reduced by either 1 microM apamin (a selective small conductance Ca(2+)-sensitive K+ channel, SKCa, inhibitor) or the selective L-type Ca2+ channel inhibitor, nicardipine (3 microM). 5 SNP-induced relaxations were insensitive to 1 mM tetraethylammonium chloride (an inhibitor of large-conductance Ca(2+)-sensitive K+ channels) and 1 microM glibenclamide (an ATP-sensitive K+ channel inhibitor). 6 These data suggest that SNP-mediated fundus relaxation occurs via a cGMP-mediated and ryanodine-sensitive mechanism which requires, at least in part, SKCa and L-type Ca2+ channel activity.

Item Type: Article
Authors :
NameEmailORCID
Geeson, JUNSPECIFIEDUNSPECIFIED
Larsson, KUNSPECIFIEDUNSPECIFIED
Hourani, SMs.hourani@surrey.ac.ukUNSPECIFIED
Toms, NJUNSPECIFIEDUNSPECIFIED
Date : October 2002
Uncontrolled Keywords : Animals, Apamin, Calcium Channel Blockers, Calcium Channels, L-Type, Carbachol, Cyclic GMP, Enzyme Inhibitors, Gastric Fundus, Guanylate Cyclase, In Vitro Techniques, Male, Muscle Contraction, Muscle Relaxation, Muscle, Smooth, Nicardipine, Nitric Oxide, Nitroprusside, Oxadiazoles, Parasympathomimetics, Potassium Channel Blockers, Potassium Channels, Calcium-Activated, Quinoxalines, Rats, Rats, Wistar, Ryanodine, Tetraethylammonium
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:16
Last Modified : 17 May 2017 14:41
URI: http://epubs.surrey.ac.uk/id/eprint/823014

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