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Modulation of intestinal gene expression by dietary zinc status: effectiveness of cDNA arrays for expression profiling of a single nutrient deficiency.

Blanchard, RK, Moore, JB, Green, CL and Cousins, RJ (2001) Modulation of intestinal gene expression by dietary zinc status: effectiveness of cDNA arrays for expression profiling of a single nutrient deficiency. Proc Natl Acad Sci U S A, 98 (24). pp. 13507-13513.

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Abstract

Mammalian nutritional status affects the homeostatic balance of multiple physiological processes and their associated gene expression. Although DNA array analysis can monitor large numbers of genes, there are no reports of expression profiling of a micronutrient deficiency in an intact animal system. In this report, we have tested the feasibility of using cDNA arrays to compare the global changes in expression of genes of known function that occur in the early stages of rodent zinc deficiency. The gene-modulating effects of this deficiency were demonstrated by real-time quantitative PCR measurements of altered mRNA levels for metallothionein 1, zinc transporter 2, and uroguanylin, all of which have been previously documented as zinc-regulated genes. As a result of the low level of inherent noise within this model system and application of a recently reported statistical tool for statistical analysis of microarrays [Tusher, V.G., Tibshirani, R. & Chu, G. (2001) Proc. Natl. Acad. Sci. USA 98, 5116-5121], we demonstrate the ability to reproducibly identify the modest changes in mRNA abundance produced by this single micronutrient deficiency. Among the genes identified by this array profile are intestinal genes that influence signaling pathways, growth, transcription, redox, and energy utilization. Additionally, the influence of dietary zinc supply on the expression of some of these genes was confirmed by real-time quantitative PCR. Overall, these data support the effectiveness of cDNA array expression profiling to investigate the pleiotropic effects of specific nutrients and may provide an approach to establishing markers for assessment of nutritional status.

Item Type: Article
Authors :
NameEmailORCID
Blanchard, RKUNSPECIFIEDUNSPECIFIED
Moore, JBj.b.moore@surrey.ac.ukUNSPECIFIED
Green, CLUNSPECIFIEDUNSPECIFIED
Cousins, RJUNSPECIFIEDUNSPECIFIED
Date : 20 November 2001
Identification Number : https://doi.org/10.1073/pnas.251532498
Uncontrolled Keywords : Animals, Dietary Supplements, Gene Expression Profiling, Gene Expression Regulation, Intestines, Male, Oligonucleotide Array Sequence Analysis, Rats, Rats, Sprague-Dawley, Zinc
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:15
Last Modified : 17 May 2017 14:41
URI: http://epubs.surrey.ac.uk/id/eprint/822948

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