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Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium.

Summerfield, N, Peters, ME, Hercock, CA, Mobasheri, A and Young, IS (2010) Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium. J Vet Cardiol, 12 (1). pp. 17-23.

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Abstract

OBJECTIVES: Dilated cardiomyopathy (DCM) is one of the most common acquired canine heart diseases. It is particularly common in large and giant breed dogs. Although a great deal is known about the clinical progression and manifestations of the disease, the underlying cellular and molecular mechanisms remain poorly understood. One widely held belief is that calcium-handling abnormalities are critically involved in the disease process. This study investigates the changes in expression of the sarco(endo)plasmic reticulum calcium ATPase (SERCA) isoforms in DCM myocardium from German shepherd dogs. ANIMALS, MATERIALS AND METHODS: Affected tissue samples were obtained from German shepherd dogs with DCM, euthanized for intractable congestive heart failure while normal myocardial tissue samples were obtained from German shepherd dogs, euthanized for non-cardiovascular reasons. Tissue microarrays containing normal and DCM myocardium samples were prepared, immunostained with SERCA1 and SERCA2 antibodies and analyzed. RESULTS: We were able to demonstrate, for the first time, that while there is little change in the expression of the cardiac isoform (SERCA2), there is clear expression of the fast-twitch skeletal muscle isoform SERCA1 in the myocardium of dogs diagnosed with DCM. CONCLUSION: We propose that SERCA1 expression is evidence of a natural adaptive response to the impaired Ca2+ handling thought to occur in German shepherd dogs with DCM and heart failure.

Item Type: Article
Authors :
NameEmailORCID
Summerfield, NUNSPECIFIEDUNSPECIFIED
Peters, MEUNSPECIFIEDUNSPECIFIED
Hercock, CAUNSPECIFIEDUNSPECIFIED
Mobasheri, Aa.mobasheri@surrey.ac.ukUNSPECIFIED
Young, ISUNSPECIFIEDUNSPECIFIED
Date : April 2010
Identification Number : https://doi.org/10.1016/j.jvc.2009.12.001
Uncontrolled Keywords : Animals, Cardiomyopathy, Dilated, Dog Diseases, Dogs, Endoplasmic Reticulum, Heart Ventricles, Male, Myocardium, Protein Array Analysis, Sarcoplasmic Reticulum Calcium-Transporting ATPases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:03
Last Modified : 17 May 2017 14:39
URI: http://epubs.surrey.ac.uk/id/eprint/822048

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