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Construction and characterization of an oncolytic HSV vector containing a fusogenic glycoprotein and prodrug activation for enhanced local tumor control.

Simpson, GR and Coffin, RS (2009) Construction and characterization of an oncolytic HSV vector containing a fusogenic glycoprotein and prodrug activation for enhanced local tumor control. Methods Mol Biol, 542. pp. 551-564.

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Abstract

A large number of oncolytic viral vectors are currently under clinical development for cancer therapy. Herpes simplex virus type 1 (HSV-1) has demonstrated particular promise in this field, showing genetically engineered selective tumor replication and cytotoxicity in a wide variety of tumor types, without damaging healthy tissues. Enhanced activity has been observed when a range of therapeutic genes has been inserted into various oncolytic HSV genomes. Here, we discuss methods used to develop and characterize an oncolytic HSV virus that combines expression of a highly potent prodrug activating gene (yeast cytosine deaminase/uracil phosphoribosyltransferase fusion [Fcy::Fur]) and the fusogenic glycoprotein from gibbon ape leukemia virus (GALV) for enhanced local tumor control.

Item Type: Article
Authors :
NameEmailORCID
Simpson, GRg.simpson@surrey.ac.ukUNSPECIFIED
Coffin, RSUNSPECIFIEDUNSPECIFIED
Date : 2009
Identification Number : 10.1007/978-1-59745-561-9_29
Uncontrolled Keywords : Animals, Cell Death, Cell Line, Tumor, Cloning, Molecular, Genetic Vectors, Glycoproteins, Humans, Molecular Biology, Neoplasms, Oncolytic Viruses, Plasmids, Prodrugs, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Simplexvirus
Depositing User : Symplectic Elements
Date Deposited : 17 May 2017 09:01
Last Modified : 17 May 2017 14:39
URI: http://epubs.surrey.ac.uk/id/eprint/821928

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