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Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses

Varjak, M, Maringer, Kevin, Watson, M, Sreenu, VB, Fredericks, AC, Pondeville, E, Donald, CL, Sterk, J, Kean, J, Vazeille, M , Failloux, A-B, Kohl, A and Schnettler, E (2017) Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses mSphere, 2 (3), e00144-17.

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Abstract

The small interfering RNA (siRNA) pathway is a major antiviral response in mosquitoes; however, another RNA interference pathway, the PIWI-interacting RNA (piRNA) pathway, has been suggested to be antiviral in mosquitoes. Piwi4 has been reported to be a key mediator of this response in mosquitoes, but it is not involved in the production of virus-specific piRNAs. Here, we show that Piwi4 associates with members of the antiviral exogenous siRNA pathway (Ago2 and Dcr2), as well as with proteins of the piRNA pathway (Ago3, Piwi5, and Piwi6) in an Aedes aegypti-derived cell line, Aag2. Analysis of small RNAs captured by Piwi4 revealed that it is predominantly associated with virus-specific siRNAs in Semliki Forest virusinfected cells and, to a lesser extent, with viral piRNAs. By using a Dcr2 knockout cell line, we showed directly that Ago2 lost its antiviral activity, as it was no longer bound to siRNAs, but Piwi4 retained its antiviral activity in the absence of the siRNA pathway. These results demonstrate a complex interaction between the siRNA and piRNA pathways in A. aegypti and identify Piwi4 as a noncanonical PIWI protein that interacts with members of the siRNA and piRNA pathways, and its antiviral activities may be independent of either pathway.

Item Type: Article
Subjects : Biosciences and Medicine
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Varjak, MUNSPECIFIEDUNSPECIFIED
Maringer, Kevink.maringer@surrey.ac.ukUNSPECIFIED
Watson, MUNSPECIFIEDUNSPECIFIED
Sreenu, VBUNSPECIFIEDUNSPECIFIED
Fredericks, ACUNSPECIFIEDUNSPECIFIED
Pondeville, EUNSPECIFIEDUNSPECIFIED
Donald, CLUNSPECIFIEDUNSPECIFIED
Sterk, JUNSPECIFIEDUNSPECIFIED
Kean, JUNSPECIFIEDUNSPECIFIED
Vazeille, MUNSPECIFIEDUNSPECIFIED
Failloux, A-BUNSPECIFIEDUNSPECIFIED
Kohl, AUNSPECIFIEDUNSPECIFIED
Schnettler, EUNSPECIFIEDUNSPECIFIED
Date : 3 May 2017
Identification Number : 10.1128/mSphere.00144-17
Copyright Disclaimer : Copyright © 2017 Varjak et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Uncontrolled Keywords : Aedes aegypti, PIWI, RNA interference, antiviral response, arbovirus, innate immunity
Depositing User : Symplectic Elements
Date Deposited : 09 May 2017 17:17
Last Modified : 31 Oct 2017 19:21
URI: http://epubs.surrey.ac.uk/id/eprint/814142

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