University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Antagonism of human CC-chemokine receptor 4 can be achieved through three distinct binding sites on the receptor

Slack, RJ, Russell, LJ, Barton, NP, Weston, C, Nalesso, G, Thompson, S-A, Allen, M, Chen, YH, Barnes, A, Hodgson, ST and Hall, DA (2013) Antagonism of human CC-chemokine receptor 4 can be achieved through three distinct binding sites on the receptor Pharmacology Research and Perspectives, 1 (2). e00019-1-e00019-25.

[img]
Preview
Text
Antagonism of human CC chemokine receptor.pdf - Version of Record
Available under License Creative Commons Attribution.

Download (926kB) | Preview
[img]
Preview
Text (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview

Abstract

Chemokine receptor antagonists appear to access two distinct binding sites on different members of this receptor family. One class of CCR4 antagonists has been suggested to bind to a site accessible from the cytoplasm while a second class did not bind to this site. In this report, we demonstrate that antagonists representing a variety of structural classes bind to two distinct allosteric sites on CCR4. The effects of pairs of low-molecular weight and/or chemokine CCR4 antagonists were evaluated on CCL17- and CCL22-induced responses of human CCR4+ T cells. This provided an initial grouping of the antagonists into sets which appeared to bind to distinct binding sites. Binding studies were then performed with radioligands from each set to confirm these groupings. Some novel receptor theory was developed to allow the interpretation of the effects of the antagonist combinations. The theory indicates that, generally, the concentration-ratio of a pair of competing allosteric modulators is maximally the sum of their individual effects while that of two modulators acting at different sites is likely to be greater than their sum. The low-molecular weight antagonists could be grouped into two sets on the basis of the functional and binding experiments. The antagonistic chemokines formed a third set whose behaviour was consistent with that of simple competitive antagonists. These studies indicate that there are two allosteric regulatory sites on CCR4.

Item Type: Article
Subjects : Veterinary Medicine
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Slack, RJUNSPECIFIEDUNSPECIFIED
Russell, LJUNSPECIFIEDUNSPECIFIED
Barton, NPUNSPECIFIEDUNSPECIFIED
Weston, CUNSPECIFIEDUNSPECIFIED
Nalesso, GUNSPECIFIEDUNSPECIFIED
Thompson, S-AUNSPECIFIEDUNSPECIFIED
Allen, MUNSPECIFIEDUNSPECIFIED
Chen, YHUNSPECIFIEDUNSPECIFIED
Barnes, AUNSPECIFIEDUNSPECIFIED
Hodgson, STUNSPECIFIEDUNSPECIFIED
Hall, DAUNSPECIFIEDUNSPECIFIED
Date : December 2013
Identification Number : https://doi.org/10.1002/prp2.19
Copyright Disclaimer : © 2013 GSK group of companies. All rights reserved. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.
Depositing User : Symplectic Elements
Date Deposited : 07 Apr 2017 12:13
Last Modified : 07 Apr 2017 12:13
URI: http://epubs.surrey.ac.uk/id/eprint/813987

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800